Overview
- External ontology
- mediadive.medium:J1048 (source link unavailable) — METHANOGEN HIGH SALT MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1048
Composition
flowchart LR
CultureMech_000287["methanogen_high_salt_medium"]
ing0_NaCl["NaCl"]
CultureMech_000287 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000287 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_NaHCO3["NaHCO3"]
CultureMech_000287 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_NH4Cl["NH4Cl"]
CultureMech_000287 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KCl["KCl"]
CultureMech_000287 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_KH2PO4["KH2PO4"]
CultureMech_000287 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000287 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Resazurin["Resazurin"]
CultureMech_000287 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Sodium_acetate["Sodium acetate"]
CultureMech_000287 --> ing8_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing8_Sodium_acetate -.-> CHEBI_32954
ing9_Yeast_extract["Yeast extract"]
CultureMech_000287 --> ing9_Yeast_extract
ing10_Methanol["Methanol"]
CultureMech_000287 --> ing10_Methanol
CHEBI_17790["CHEBI:17790"]
ing10_Methanol -.-> CHEBI_17790
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000287 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000287 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000287 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2SeO3["Na2SeO3"]
CultureMech_000287 --> ing14_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing14_Na2SeO3 -.-> CHEBI_48843
ing15_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000287 --> ing15_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing15_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing16_Biotin["Biotin"]
CultureMech_000287 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_Folic_acid["Folic acid"]
CultureMech_000287 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000287 --> ing18_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing19_Thiamine_HCl["Thiamine HCl"]
CultureMech_000287 --> ing19_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing19_Thiamine_HCl -.-> CHEBI_49105
ing20_Riboflavin["Riboflavin"]
CultureMech_000287 --> ing20_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing20_Riboflavin -.-> CHEBI_17015
ing21_Nicotinic_acid["Nicotinic acid"]
CultureMech_000287 --> ing21_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing21_Nicotinic_acid -.-> CHEBI_15940
ing22_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000287 --> ing22_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_Calcium_pantothenate -.-> CHEBI_31345
ing23_Vitamin_B12["Vitamin B12"]
CultureMech_000287 --> ing23_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing23_Vitamin_B12 -.-> CHEBI_176843
ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000287 --> ing24_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
ing25_Lipoic_acid["Lipoic acid"]
CultureMech_000287 --> ing25_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing25_Lipoic_acid -.-> CHEBI_16494
ing26_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000287 --> ing26_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing26_Nitrilotriacetic_acid -.-> CHEBI_44557
ing27_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000287 --> ing27_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing27_MgSO4_x_7_H2O -.-> CHEBI_31795
ing28_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_000287 --> ing28_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing28_MnSO4_x_n_H2O -.-> CHEBI_86360
ing29_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000287 --> ing29_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing29_FeSO4_x_7_H2O -.-> CHEBI_75836
CultureMech_000287 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
23.4568 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 22.4568, 1.0] |
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10.5566 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.64683 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.959693 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.959693 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.652591 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.38790800000000003 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.287908, 0.1] |
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000959693 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.959693 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 10 | Yeast extract | 1.91939 G_PER_L | ||
| 11 |
Methanol
CH4O 32.042 g/mol
|
10 G_PER_L |
CHEBI:17790
methanol
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.001 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 16 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 19 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 20 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 21 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 22 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 23 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 24 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 25 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 26 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 27 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 28 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 29 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 30 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 31 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 32 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 33 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 34 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 35 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 36 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components, except NaHCO3, and adjust pH to 7.0. Bring to a boil for 5-10 sec, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. After autoclaving, stand the medium overnight. Prior to use, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
- Comment: For strain JCM 30226, supplement with 20 mM (final) tetramethylammonium as a substrate instead of methanol.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanogen_high_salt_medium (CultureMech:000287)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.440806Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1048 |
| 2026-03-13T03:09:46.796715Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:18:54.148944Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000287 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.093114+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOGEN HIGH SALT MEDIUM" to "methanogen_high_salt_medium" for consistent matching |
| 2026-05-16T09:02:57.392423+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.671871+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.223147+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:30.374511+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 05a62aba63781ca08a65dcd41e187db9efa013b16df6a454e6ea40e91e38596a |