Overview
- External ontology
- mediadive.medium:J457 (source link unavailable) — ARTIFICIAL SEAWATER FOR HALOPHILES
- Applications
-
- Microbial cultivation
- pH
- 7.4
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=457
Composition
flowchart LR
CultureMech_000298["artificial_seawater_for_halophiles"]
ing0_NaCl["NaCl"]
CultureMech_000298 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000298 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000298 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_000298 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaHCO3["NaHCO3"]
CultureMech_000298 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_NaNO3["NaNO3"]
CultureMech_000298 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000298 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_KH2PO4["KH2PO4"]
CultureMech_000298 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_NH4Cl["NH4Cl"]
CultureMech_000298 --> ing8_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing8_NH4Cl -.-> CHEBI_31206
ing9_Yeast_extract["Yeast extract"]
CultureMech_000298 --> ing9_Yeast_extract
ing10_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_000298 --> ing10_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing10_Sodium_pyruvate -.-> CHEBI_50144
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
195 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
35 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
50 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.03 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 10 | Yeast extract | 0.05 G_PER_L | ||
| 11 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Preparation
- Add components except NaHCO3 and sodium pyruvate to distilled water and bring volume to 1.0 L. Adjust pH to 7.4 with 1 M Tris base and autoclave. Filter-sterilize 8% (w/v) NaHCO3 and 25% (w/v) sodium pyruvate solutions and add to the medium aseptically.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- artificial_seawater_for_halophiles (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.665039Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J457 |
| 2026-03-13T06:18:54.273674Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000298 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.232229+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ARTIFICIAL SEAWATER FOR HALOPHILES" to "artificial_seawater_for_halophiles" for consistent matching |
| 2026-05-16T09:02:57.222626+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.488041+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.160569+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.384100+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8278fa6adfb30f4a7d2322c08cf9c5435365077b782482f87ae9b095c43b9404 |