Overview
- External ontology
- mediadive.medium:J599 (source link unavailable) — MODERATE HALOPHILE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=599
Composition
flowchart LR
CultureMech_000301["moderate_halophile_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000301 --> ing0_Yeast_extract
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_000301 --> ing1_Proteose_peptone_no__3
ing2_Glucose["Glucose"]
CultureMech_000301 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_NaCl["NaCl"]
CultureMech_000301 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000301 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000301 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000301 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_KCl["KCl"]
CultureMech_000301 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_000301 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_NaBr["NaBr"]
CultureMech_000301 --> ing9_NaBr
CHEBI_63004["CHEBI:63004"]
ing9_NaBr -.-> CHEBI_63004
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 10 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 5 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
81 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
9.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
7 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.36 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.06 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
NaBr
Br.Na 102.894 g/mol
|
0.026 G_PER_L |
CHEBI:63004
sodium bromide
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2. For preparation of solid medium, add 20.0 g/L agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J599_moderate_halophile_medium (CultureMech:000301)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.046784Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J599 |
| 2026-03-13T06:18:54.285155Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000301 |
| 2026-03-17T17:06:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.244617+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODERATE HALOPHILE MEDIUM" to "moderate_halophile_medium" for consistent matching |
| 2026-05-16T09:02:57.678817+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | category->lowercase=1 |
| 2026-06-05T07:40:14.970025+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.336425+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-07-29T02:09:00.657591Z | domain-growth-evidence | ANNOTATED | Moved archaea/ -> bacterial/ and restamped category on growth evidence: kg-microbe records only bacterial taxa growing in this medium (Salinicoccus hispanicus, Salinicoccus roseus). The name carries a physiology (halophile/thermophile) that spans both domains, so the previous archaea/ placement was unsubstantiated (#138). Filename disambiguated with the source id because a DIFFERENT medium already occupies the original name in bacterial/ (#116). |
| 2026-08-06T03:04:36.983213+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 80c8d3c7ebd1524af118342280564bb7a26cff2e00641aa72df6654de7b259dc |