Overview
- External ontology
- mediadive.medium:C110 (source link unavailable) — ASN-III
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.atcc.org/~/media/D3547D63F887423DAFB39841A5B588B9.ashx
Composition
flowchart LR
CultureMech_000317["asn_iii"]
ing0_NaCl["NaCl"]
CultureMech_000317 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000317 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000317 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000317 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_000317 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_Citric_acid["Citric acid"]
CultureMech_000317 --> ing5_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing5_Citric_acid -.-> CHEBI_30769
ing6_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_000317 --> ing6_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing6_Ferric_ammonium_citrate -.-> CHEBI_31604
ing7_EDTA["EDTA"]
CultureMech_000317 --> ing7_EDTA
CHEBI_4735["CHEBI:4735"]
ing7_EDTA -.-> CHEBI_4735
ing8_NaNO3["NaNO3"]
CultureMech_000317 --> ing8_NaNO3
CHEBI_63005["CHEBI:63005"]
ing8_NaNO3 -.-> CHEBI_63005
ing9_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_000317 --> ing9_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing10_Na2CO3["Na2CO3"]
CultureMech_000317 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_Vitamin_B12["Vitamin B12"]
CultureMech_000317 --> ing11_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing11_Vitamin_B12 -.-> CHEBI_176843
ing12_Agar["Agar"]
CultureMech_000317 --> ing12_Agar
CHEBI_2509["CHEBI:2509"]
ing12_Agar -.-> CHEBI_2509
ing13_H3BO3["H3BO3"]
CultureMech_000317 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000317 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000317 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000317 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000317 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_000317 --> ing18_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing18_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
25 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
Citric acid
C6H8O7 192.123 g/mol
|
0.003 G_PER_L |
CHEBI:30769
citric acid
|
|
| 7 | Ferric ammonium citrate | 0.003 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 8 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.0005 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 9 |
NaNO3
NO3.Na 84.994 g/mol
|
0.75 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 10 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
0.75 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.02 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 13 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.039 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.079 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.049 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Preparation
- Adjust pH to 7.3. Filter-sterilize and dispense into appropriate vessel.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- asn_iii (CultureMech:000317)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.833816Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C110 |
| 2026-03-13T06:18:54.403628Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000317 |
| 2026-03-17T06:30:06.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.374769+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ASN-III" to "asn_iii" for consistent matching |
| 2026-06-05T07:40:49.870480+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.582631+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.153647+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 854bfc12baa18cb5e63b6e167609d9004373552c16f9c0bc6ed24bf3055c3c18 |