asw_ses

Overview

External ontology

mediadive.medium:C11 (source link unavailable) — ASW:SES

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_ASW_SES.pdf

Composition

flowchart LR
    CultureMech_000318["asw_ses"]
    ing0_Tricine["Tricine"]
    CultureMech_000318 --> ing0_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing0_Tricine -.-> CHEBI_46760
    ing1_Natural_sea_water["Natural sea water"]
    CultureMech_000318 --> ing1_Natural_sea_water
    ing2_NaNO3["NaNO3"]
    CultureMech_000318 --> ing2_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing2_NaNO3 -.-> CHEBI_63005
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_000318 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683
    ing4_K2HPO4["K2HPO4"]
    CultureMech_000318 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Biotin["Biotin"]
    CultureMech_000318 --> ing5_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing5_Biotin -.-> CHEBI_15956
    ing6_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000318 --> ing6_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing6_Calcium_pantothenate -.-> CHEBI_31345
    ing7_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000318 --> ing7_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing7_Cyanocobalamine -.-> CHEBI_17439
    ing8_Folic_acid["Folic acid"]
    CultureMech_000318 --> ing8_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing8_Folic_acid -.-> CHEBI_27470
    ing9_Inositol["Inositol"]
    CultureMech_000318 --> ing9_Inositol
    CHEBI_24848["CHEBI:24848"]
    ing9_Inositol -.-> CHEBI_24848
    ing10_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000318 --> ing10_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing10_Nicotinic_acid -.-> CHEBI_15940
    ing11_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000318 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_Thymine["Thymine"]
    CultureMech_000318 --> ing12_Thymine
    CHEBI_17821["CHEBI:17821"]
    ing12_Thymine -.-> CHEBI_17821
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000318 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_KNO3["KNO3"]
    CultureMech_000318 --> ing14_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing14_KNO3 -.-> CHEBI_63043
    ing15_Soil["Soil"]
    CultureMech_000318 --> ing15_Soil

Ingredients (16)

Preparation

1. 3:1 mixture
2. See separate recipes ASW and SES. This medium is made up in 2 parts which are mixed before autoclaving.
3. Make up to 1 litre with filtered natural sea water. Adjust the pH to 7.6 – 7.8 with 1N NaOH or 1N HCl. Autoclave at 15 psi.
4. Alternatively use "Ultramarine Synthetica" sea salts 33.6 g and make up to 1 litre with distilled water. Adjust pH as above
5. Make up to 1 litre with deionized water and autoclave at 15 psi for 15 minutes.
6. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
7. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
8. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• asw_ses (normalized source unresolved)