bb_merds

Overview

External ontology

mediadive.medium:C16 (source link unavailable) — BB:MErds

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_BB_MErds.pdf

Composition

flowchart LR
    CultureMech_000323["bb_merds"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000323 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000323 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_000323 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000323 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000323 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000323 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_H3BO3["H3BO3"]
    CultureMech_000323 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_EDTA["EDTA"]
    CultureMech_000323 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_KOH["KOH"]
    CultureMech_000323 --> ing8_KOH
    CHEBI_32035["CHEBI:32035"]
    ing8_KOH -.-> CHEBI_32035
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000323 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_H2SO4["H2SO4"]
    CultureMech_000323 --> ing10_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing10_H2SO4 -.-> CHEBI_26836
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000323 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000323 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_MoO3["MoO3"]
    CultureMech_000323 --> ing13_MoO3
    CHEBI_30627["CHEBI:30627"]
    ing13_MoO3 -.-> CHEBI_30627
    ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000323 --> ing14_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing15_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000323 --> ing15_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing15_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing16_Na2HPO4["Na2HPO4"]
    CultureMech_000323 --> ing16_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing16_Na2HPO4 -.-> CHEBI_34683
    ing17_Sea_water["Sea water"]
    CultureMech_000323 --> ing17_Sea_water
    ing18_KNO3["KNO3"]
    CultureMech_000323 --> ing18_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing18_KNO3 -.-> CHEBI_63043
    ing19_Soil["Soil"]
    CultureMech_000323 --> ing19_Soil

Ingredients (20)

Preparation

1. 8:2 mixture or 1:1 mixture
2. See separate recipes. Mix then autoclave at 15 psi for 15 minutes.
3. Make up to 1 litre with glass distilled or deionised water.
4. Mix the above constituents and autoclave at 15 psi for 15 minutes. It may be necessary to filter final medium to avoid problems with precipitate.
5. Make up to 1 litre with deionized water and autoclave at 15 psi for 15 minutes.
6. At the CCAP, SE1 is used for marine algae, SE2 for freshwater and terrestrial protozoa
7. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
8. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
9. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.
10. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
11. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2­4 mm) or stored as it is prior to use.
12. Soil is prepared as above. 105 g of air­dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes, then again after 24 hours. The contents of the bottle are left to settle (usually for at least a week) and then the supernatant is decanted and filtered. The final pH should be 7.0 - 8.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• bb_merds (normalized source unresolved)