ea

Overview

External ontology

mediadive.medium:C33 (source link unavailable) — EA

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_EA.pdf

Composition

flowchart LR
    CultureMech_000342["ea"]
    ing0_KNO3["KNO3"]
    CultureMech_000342 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000342 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NaCl["NaCl"]
    CultureMech_000342 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_000342 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000342 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_000342 --> ing5_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing6_Agar["Agar"]
    CultureMech_000342 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Soil["Soil"]
    CultureMech_000342 --> ing7_Soil

Ingredients (8)

Preparation

1. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
3. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• ea (normalized source unresolved)