Overview
- External ontology
- mediadive.medium:C38 (source link unavailable) — f/2 + Si for heterotrophic growth
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_f2Si_heterotrophic.pdf
Composition
flowchart LR
CultureMech_000347["f_2_si_for_heterotrophic_growth"]
ing0_NaNO3["NaNO3"]
CultureMech_000347 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
CultureMech_000347 --> ing1_NaH2PO4_x_2_H2O
CHEBI_37585["CHEBI:37585"]
ing1_NaH2PO4_x_2_H2O -.-> CHEBI_37585
ing2_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
CultureMech_000347 --> ing2_Na2SiO3_x_9_H2O
CHEBI_132108["CHEBI:132108"]
ing2_Na2SiO3_x_9_H2O -.-> CHEBI_132108
ing3_Natural_sea_water["Natural sea water"]
CultureMech_000347 --> ing3_Natural_sea_water
ing4_Na2_EDTA["Na2-EDTA"]
CultureMech_000347 --> ing4_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing4_Na2_EDTA -.-> CHEBI_64734
ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000347 --> ing5_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000347 --> ing6_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000347 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000347 --> ing8_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000347 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000347 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing11_Cyanocobalamine["Cyanocobalamine"]
CultureMech_000347 --> ing11_Cyanocobalamine
CHEBI_17439["CHEBI:17439"]
ing11_Cyanocobalamine -.-> CHEBI_17439
ing12_Thiamine_HCl["Thiamine HCl"]
CultureMech_000347 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Biotin["Biotin"]
CultureMech_000347 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
|
1 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 3 |
Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
|
1 G_PER_L |
CHEBI:132108
sodium silicate nonahydrate
|
|
| 4 | Natural sea water | 1000 G_PER_L | ||
| 5 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
4.16 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 6 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
3.15 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 7 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.01 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.18 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.006 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 12 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
0.0005 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
|
| 13 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0005 G_PER_L |
CHEBI:15956
biotin
|
Preparation
- Add while stirring:
- Silicate can react with glass, ensure this stock solution is stored in plastic bottles.
- Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or HCl. Sterilise by autoclaving for 15 minutes at 15 psi and use when cooled to room temperature.
- For heterotrophic growth (in the dark) add either 0.5 g/l yeast extract and 5 g/l glucose or 0.5 g/l yeast extract and 3 g/l sodium acetate.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- f_2_si_for_heterotrophic_growth (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.598971Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C38 |
| 2026-03-13T06:18:54.640663Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000347 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.634150+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "f/2 + Si for heterotrophic growth" to "f_2_si_for_heterotrophic_growth" for consistent matching |
| 2026-06-05T07:40:59.831828+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:33.831225+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 66acc10874cb01a48d52fa209c8afee19dee11374533fc288b8c76b53840c719 |