Overview
- External ontology
- mediadive.medium:C3 (source link unavailable) — ANT
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.6, 'max': 7.8}
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_ANT.pdf
Composition
flowchart LR
CultureMech_000349["ant"]
ing0_KNO3["KNO3"]
CultureMech_000349 --> ing0_KNO3
CHEBI_63043["CHEBI:63043"]
ing0_KNO3 -.-> CHEBI_63043
ing1_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
CultureMech_000349 --> ing1_NaH2PO4_x_2_H2O
CHEBI_37585["CHEBI:37585"]
ing1_NaH2PO4_x_2_H2O -.-> CHEBI_37585
ing2_Tris["Tris"]
CultureMech_000349 --> ing2_Tris
CHEBI_9754["CHEBI:9754"]
ing2_Tris -.-> CHEBI_9754
ing3_Glycine["Glycine"]
CultureMech_000349 --> ing3_Glycine
CHEBI_15428["CHEBI:15428"]
ing3_Glycine -.-> CHEBI_15428
ing4_Thiamine_HCl["Thiamine HCl"]
CultureMech_000349 --> ing4_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing4_Thiamine_HCl -.-> CHEBI_49105
ing5_Cyanocobalamine["Cyanocobalamine"]
CultureMech_000349 --> ing5_Cyanocobalamine
CHEBI_17439["CHEBI:17439"]
ing5_Cyanocobalamine -.-> CHEBI_17439
ing6_Biotine["Biotine"]
CultureMech_000349 --> ing6_Biotine
CHEBI_15956["CHEBI:15956"]
ing6_Biotine -.-> CHEBI_15956
ing7_Natural_sea_water["Natural sea water"]
CultureMech_000349 --> ing7_Natural_sea_water
ing8_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_000349 --> ing8_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing8_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000349 --> ing9_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
ing10_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_000349 --> ing10_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing10_MnSO4_x_4_H2O -.-> CHEBI_86358
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000349 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000349 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000349 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_000349 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO3
K.NO3 101.102 g/mol
|
0.05 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 2 |
NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
|
0.0078 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 3 |
Tris
C4H11NO3 121.136 g/mol
|
1 G_PER_L |
CHEBI:9754
tris
|
|
| 4 |
Glycine
C2H5NO2 75.067 g/mol
|
0.3 G_PER_L |
CHEBI:15428
glycine
|
|
| 5 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
500 MICROG_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 6 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
2 MICROG_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
|
| 7 |
Biotine
C10H16N2O3S 244.316 g/mol
|
1 MICROG_PER_L |
CHEBI:15956
biotin
|
|
| 8 | Natural sea water | 800 G_PER_L | ||
| 9 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
3.24 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 10 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.08 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 11 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.45 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.23 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.097 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.0056 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Preparation
- Make up to 1 litre with distilled water and autoclave at 15 psi. Final pH should be 7.6 - 7.8.
- Make up to 1 litre with distilled water and adjust pH to 7.6 - 7.8 with dilute HCl or NaOH. Store frozen.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ant (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.477892Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C3 |
| 2026-03-13T06:18:54.653399Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000349 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.647584+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANT" to "ant" for consistent matching |
| 2026-05-20T06:01:08.846565+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.6-7.8' -> {'min': 7.6, 'max': 7.8} |
| 2026-06-05T07:40:49.346089+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.523560+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:53.398119+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:23.767495+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ea9762ae7b92bcab70995261c59efa546314eb121820f38e8ee11ffc0b892736 |