Overview
- External ontology
- mediadive.medium:C42 (source link unavailable) — K Medium
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_K.pdf
Composition
flowchart LR
CultureMech_000352["k_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_000352 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1__NH4_Cl["(NH4)Cl"]
CultureMech_000352 --> ing1__NH4_Cl
CHEBI_31206["CHEBI:31206"]
ing1__NH4_Cl -.-> CHEBI_31206
ing2_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
CultureMech_000352 --> ing2_Sodium_beta_glycerophosphate
CHEBI_132089["CHEBI:132089"]
ing2_Sodium_beta_glycerophosphate -.-> CHEBI_132089
ing3_H2SeO3["H2SeO3"]
CultureMech_000352 --> ing3_H2SeO3
CHEBI_26642["CHEBI:26642"]
ing3_H2SeO3 -.-> CHEBI_26642
ing4_Tris_base["Tris base"]
CultureMech_000352 --> ing4_Tris_base
CHEBI_9754["CHEBI:9754"]
ing4_Tris_base -.-> CHEBI_9754
ing5_Na2_EDTA["Na2-EDTA"]
CultureMech_000352 --> ing5_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing5_Na2_EDTA -.-> CHEBI_64734
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000352 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000352 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000352 --> ing8_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000352 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000352 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000352 --> ing11_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing12_Thiamine_HCl["Thiamine HCl"]
CultureMech_000352 --> ing12_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing12_Thiamine_HCl -.-> CHEBI_49105
ing13_Cyanocobalamin["Cyanocobalamin"]
CultureMech_000352 --> ing13_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing13_Cyanocobalamin -.-> CHEBI_17439
ing14_Biotin["Biotin"]
CultureMech_000352 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
(NH4)Cl
Cl.H4N 53.492 g/mol
|
1.5274 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
|
1 G_PER_L |
CHEBI:132089
sodium glycerol 2-phosphate
|
|
| 4 |
H2SeO3
H2O3Se 128.973 g/mol
|
0.03004 G_PER_L |
CHEBI:26642
selenous acid
|
|
| 5 |
Tris base
C4H11NO3 121.136 g/mol
|
0.6181 G_PER_L |
CHEBI:9754
tris
|
|
| 6 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
43.6 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
31.5 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 13 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 14 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
|
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Preparation
- Note for Tris base: First add to 50ml of DIW and pH. Then slowly top up to 100ml with DIW ensuring to check pH remains at 7.2.
- Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or 1M HCl. Autoclave at 15 psi for 15 minutes.
- There are 7 chemicals. Prepare Primary stock solutions first. Add first three chemicals separately in 100ml/dH2O, allowing each to completely dissolve before adding the primary stocks in order:
- First make primary stocks of Cyanocobalamin and Biotin.
- Dispense any excess primary stocks into 1 ml aliquots and freeze.
- For final vitamin mix stock solution:
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- k_medium (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.613546Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C42 |
| 2026-03-13T06:18:54.678187Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000352 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.675661+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "K Medium" to "k_medium" for consistent matching |
| 2026-06-05T07:41:10.286068+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:39.471339+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a3bd9f308ccc2537e4fc3b57c221e035d392418c5dcd9a743019988ed47f47d5 |