k35

Overview

External ontology

mediadive.medium:C44 (source link unavailable) — K35

Applications

• Microbial cultivation

pH range

{'min': 8.1, 'max': 8.4}

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_K35.pdf

Composition

flowchart LR
    CultureMech_000354["k35"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_000354 --> ing0_Sea_Salt
    ing1_NaNO3["NaNO3"]
    CultureMech_000354 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2__NH4_Cl["(NH4)Cl"]
    CultureMech_000354 --> ing2__NH4_Cl
    CHEBI_31206["CHEBI:31206"]
    ing2__NH4_Cl -.-> CHEBI_31206
    ing3_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
    CultureMech_000354 --> ing3_Sodium_beta_glycerophosphate
    CHEBI_132089["CHEBI:132089"]
    ing3_Sodium_beta_glycerophosphate -.-> CHEBI_132089
    ing4_H2SeO3["H2SeO3"]
    CultureMech_000354 --> ing4_H2SeO3
    CHEBI_26642["CHEBI:26642"]
    ing4_H2SeO3 -.-> CHEBI_26642
    ing5_Tris_base["Tris base"]
    CultureMech_000354 --> ing5_Tris_base
    CHEBI_9754["CHEBI:9754"]
    ing5_Tris_base -.-> CHEBI_9754
    ing6_Agar["Agar"]
    CultureMech_000354 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_000354 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000354 --> ing8_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing8_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000354 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000354 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000354 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000354 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000354 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000354 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_000354 --> ing15_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing15_Cyanocobalamin -.-> CHEBI_17439
    ing16_Biotin["Biotin"]
    CultureMech_000354 --> ing16_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotin -.-> CHEBI_15956

Ingredients (17)

Preparation

1. Note for Tris base: First add to 50ml of DIW and pH. Then slowly top up to 100ml with DIW ensuring to check pH remains at 7.2.
2. Make up to 1 litre with deionised water. Adjust pH to 8.1-8.4 with 1M NaOH or 1M HCl prior to autoclaving. For agar add 15g per litre Bacteriological Agar. Autoclave at 15 psi for 15 minutes.
3. There are 7 chemicals. Prepare Primary stock solutions first. Add first three chemicals separately in 100ml/dH2O, allowing each to completely dissolve before adding the primary stocks in order:
4. First make primary stocks of Cyanocobalamin and Biotin.
5. Dispense any excess primary stocks into 1 ml aliquots and freeze.
6. For final vitamin mix stock solution:

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• k35 (normalized source unresolved)