masm

Overview

External ontology

mediadive.medium:C47 (source link unavailable) — MASM

Applications

• Microbial cultivation

Synonyms

• {'name': 'bw_masm', 'source': 'MediaDive', 'source_id': 'mediadive.medium:C19', 'original_category': 'bacterial'}

pH

8.0

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MASM.pdf

Composition

flowchart LR
    CultureMech_000357["masm"]
    ing0_Tris["Tris"]
    CultureMech_000357 --> ing0_Tris
    CHEBI_9754["CHEBI:9754"]
    ing0_Tris -.-> CHEBI_9754
    ing1_NaCl["NaCl"]
    CultureMech_000357 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000357 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_000357 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaNO3["NaNO3"]
    CultureMech_000357 --> ing4_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing4_NaNO3 -.-> CHEBI_63005
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000357 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KH2PO4["KH2PO4"]
    CultureMech_000357 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_NH4Cl["NH4Cl"]
    CultureMech_000357 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_Na2_EDTA["Na2-EDTA"]
    CultureMech_000357 --> ing8_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing8_Na2_EDTA -.-> CHEBI_64734
    ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000357 --> ing9_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000357 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_ZnCl2["ZnCl2"]
    CultureMech_000357 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000357 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_000357 --> ing13_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_000357 --> ing14_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_hydrochloride -.-> CHEBI_49105
    ing15_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000357 --> ing15_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing15_Cyanocobalamine -.-> CHEBI_17439
    ing16_Soil["Soil"]
    CultureMech_000357 --> ing16_Soil

Ingredients (17)

Preparation

1. Make up to 1 litre with distilled water and adjust to pH 8.0 with 1M NaOH or 1M HCl. Autoclave at 15 psi for 15 minutes.
2. For brackish organisms, take 15 g of NaCl instead of 30 g (BW/MASM).
3. Ensure elements are added in the following sequence:
4. Once elements are dissolved autoclave at 15 psi for 15 minutes.
5. Filter sterile
6. Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.
7. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
8. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
9. Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• bw_masm (CultureMech:000326)
• masm (normalized source unresolved)