Overview
- External ontology
- mediadive.medium:C50 (source link unavailable) — MC II
- Applications
-
- Microbial cultivation
- pH
- 6.9
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MCII.pdf
Composition
flowchart LR
CultureMech_000361["mc_ii"]
ing0_Casein["Casein"]
CultureMech_000361 --> ing0_Casein
ing1_Na2HPO4["Na2HPO4"]
CultureMech_000361 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2_KH2PO4["KH2PO4"]
CultureMech_000361 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Yeast_extract["Yeast extract"]
CultureMech_000361 --> ing3_Yeast_extract
ing4_D_Glucose["D-Glucose"]
CultureMech_000361 --> ing4_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing4_D_Glucose -.-> CHEBI_17634
ing5_Calf_serum["Calf serum"]
CultureMech_000361 --> ing5_Calf_serum
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein | 11.1111 G_PER_L | ||
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
1.47222 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.888889 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 | Yeast extract | 5.55556 G_PER_L | ||
| 5 |
D-Glucose
C6H12O6 180.156 g/mol
|
2.77778 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 6 | Calf serum | 20 G_PER_L |
Preparation
- To be added later:
- Add the first six constituents in the order shown to 900 ml of deionised water, allowing each to dissolve completely before adding the next. Adjust to pH 6.9. Dispense into 5 x 180 ml aliquots and sterilise by pressure cooking at 10 psi for 15 minutes.
- Aseptically add the foetal calf serum to a final concentration of 10%.
- Store at 4°C.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mc_ii (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:19.647265Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C50 |
| 2026-03-13T06:18:54.752421Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000361 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.756892+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MC II" to "mc_ii" for consistent matching |
| 2026-06-05T07:41:13.395935+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.060252+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.348817+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3213eb0cb71b7dd391efc89df40cb2662a7304e5bd02d6d0cc85362c72199ec1 |