Overview
- External ontology
- mediadive.medium:C52 (source link unavailable) — MDY-V
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MDY_V.pdf
Composition
flowchart LR
CultureMech_000363["mdy_v"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000363 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_KCl["KCl"]
CultureMech_000363 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_NH4Cl["NH4Cl"]
CultureMech_000363 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaNO3["NaNO3"]
CultureMech_000363 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
CultureMech_000363 --> ing4_Sodium_beta_glycerophosphate
CHEBI_132089["CHEBI:132089"]
ing4_Sodium_beta_glycerophosphate -.-> CHEBI_132089
ing5_H3BO3["H3BO3"]
CultureMech_000363 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_CaCl2["CaCl2"]
CultureMech_000363 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_MES["MES"]
CultureMech_000363 --> ing7_MES
CHEBI_39010["CHEBI:39010"]
ing7_MES -.-> CHEBI_39010
ing8_Agar["Agar"]
CultureMech_000363 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Na2_EDTA["Na2-EDTA"]
CultureMech_000363 --> ing9_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing9_Na2_EDTA -.-> CHEBI_64734
ing10_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000363 --> ing10_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing10_FeCl3_x_6_H2O -.-> CHEBI_86254
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000363 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_ZnCl2_x_6_H2O["ZnCl2 x 6 H2O"]
CultureMech_000363 --> ing12_ZnCl2_x_6_H2O
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000363 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000363 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_Thiamine_hydrochloride["Thiamine hydrochloride"]
CultureMech_000363 --> ing15_Thiamine_hydrochloride
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_hydrochloride -.-> CHEBI_49105
ing16_Cyanocobalamine["Cyanocobalamine"]
CultureMech_000363 --> ing16_Cyanocobalamine
CHEBI_17439["CHEBI:17439"]
ing16_Cyanocobalamine -.-> CHEBI_17439
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.5274 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 5 |
Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
|
1 G_PER_L |
CHEBI:132089
sodium glycerol 2-phosphate
|
|
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 8 | MES | 0.2 G_PER_L |
CHEBI:39010
MES
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 10 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.75 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 11 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.097 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.041 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 | ZnCl2 x 6 H2O | 0.005 G_PER_L | ||
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.002 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.004 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
|
1.2 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 17 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Preparation
- Make up to 1 litre with distilled water. Adjust pH to 6.8 with NaOH, then autoclave at 15 psi for 15 minutes. For agar add 15 g per litre Bacterial Agar.
- Add to 1000 ml of distilled water 0.75 g Na2EDTA and the minerals in exactly the following sequence:
- Filter sterile
- Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mdy_v (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.651379Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C52 |
| 2026-03-13T06:18:54.762971Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000363 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.769041+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MDY-V" to "mdy_v" for consistent matching |
| 2026-06-05T07:41:13.515031+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.082114+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.470561+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 088d3c35624e389e50d8a74bd910afff4769d11a52121cb79cabc57b81f96bd7 |