merds_my75s

Overview

External ontology

mediadive.medium:C54 (source link unavailable) — MErds/MY75S

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MErds_MY75S.pdf

Composition

flowchart LR
    CultureMech_000365["merds_my75s"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000365 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_000365 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_Sea_water["Sea water"]
    CultureMech_000365 --> ing2_Sea_water
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000365 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000365 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KNO3["KNO3"]
    CultureMech_000365 --> ing5_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing5_KNO3 -.-> CHEBI_63043
    ing6_Soil["Soil"]
    CultureMech_000365 --> ing6_Soil
    ing7_Natural_sea_water["Natural sea water"]
    CultureMech_000365 --> ing7_Natural_sea_water
    ing8_Malt_extract["Malt extract"]
    CultureMech_000365 --> ing8_Malt_extract
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_000365 --> ing9_Yeast_extract
    ing10_Agar["Agar"]
    CultureMech_000365 --> ing10_Agar
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar -.-> CHEBI_2509

Ingredients (11)

Preparation

1. Biphasic. See separate recipes.
2. Mix the above constituents and autoclave at 15 psi for 15 minutes. It may be necessary to filter final medium to avoid problems with precipitate.
3. Make up to 1 litre with deionized water and autoclave at 15 psi for 15 minutes.
4. At the CCAP, SE1 is used for marine algae, SE2 for freshwater and terrestrial protozoa
5. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
6. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
7. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.
8. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
9. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
10. Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.
11. Thoroughly disperse the agar in cold 75% seawater. Bring to the boil, stirring continuously. During heating add the other constituents. Transfer the molten agar to suitable vessels and sterilise at 15 psi for 15 minutes. If available a bench top agar maker is preferable to making this medium. The constant stirring during sterilisation minimises flocculation.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• merds_my75s (normalized source unresolved)