pe

Overview

External ontology

mediadive.medium:C74 (source link unavailable) — PE

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_PE.pdf

Composition

flowchart LR
    CultureMech_000387["pe"]
    ing0_Natural_sea_water["Natural sea water"]
    CultureMech_000387 --> ing0_Natural_sea_water
    ing1_Soil["Soil"]
    CultureMech_000387 --> ing1_Soil
    ing2_NaNO3["NaNO3"]
    CultureMech_000387 --> ing2_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing2_NaNO3 -.-> CHEBI_63005
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_000387 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683

Ingredients (4)

Preparation

1. Filter natural seawater and reduce to 95% using glass distilled water. Autoclave.
2. Ingredients above should be autoclaved separately and added together cold using aseptic techniques. Sterilise for 15 minutes at 15 psi and use when cooled to room temperature.
3. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
4. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
5. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• pe (normalized source unresolved)