Overview
- External ontology
- mediadive.medium:C77 (source link unavailable) — PJ/NN
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_PJ_NN.pdf
Composition
flowchart LR
CultureMech_000390["pj_nn"]
ing0_K2HPO4["K2HPO4"]
CultureMech_000390 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000390 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000390 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_000390 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Agar["Agar"]
CultureMech_000390 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_NaCl["NaCl"]
CultureMech_000390 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_000390 --> ing6_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing6_CaCl2_x_6_H2O -.-> CHEBI_91243
ing7_Na2HPO4["Na2HPO4"]
CultureMech_000390 --> ing7_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4 -.-> CHEBI_34683
ing8_KH2PO4["KH2PO4"]
CultureMech_000390 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 1.0, 0.8] |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
1.6 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
24 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
1.2 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 8 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
28.4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
27.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Preparation
- Biphasic. See separate recipes below.
- Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.
- Thoroughly disperse the agar in cold amoeba saline solution and then slowly bring to the boil. Transfer the molten agar to suitable vessels and sterilize at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pj_nn (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.739217Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C77 |
| 2026-03-13T03:08:59.236044Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:54.910203Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000390 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.919881+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PJ/NN" to "pj_nn" for consistent matching |
| 2026-06-05T07:41:42.059773+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.269733+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.068576+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3465738cfd00cdc9511b696c92c7de8ba95547791b6a2866c5da2023df962c98 |