Overview
- External ontology
- mediadive.medium:C83 (source link unavailable) — RPL:MP
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_RPL_MP.pdf
Composition
flowchart LR
CultureMech_000397["rpl_mp"]
ing0_Rye_grass["Rye grass"]
CultureMech_000397 --> ing0_Rye_grass
ing1_K2HPO4["K2HPO4"]
CultureMech_000397 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000397 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000397 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_000397 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_000397 --> ing5_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing5_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing6_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_000397 --> ing6_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing6_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing7_Fe_SO4_x_7_H2O["Fe SO4 x 7 H2O"]
CultureMech_000397 --> ing7_Fe_SO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_Fe_SO4_x_7_H2O -.-> CHEBI_75836
ing8_H2SO4["H2SO4"]
CultureMech_000397 --> ing8_H2SO4
CHEBI_26836["CHEBI:26836"]
ing8_H2SO4 -.-> CHEBI_26836
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rye grass | 1 G_PER_L | ||
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 1.0, 1.0] |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
2.6 G_PER_L |
CHEBI:32588
potassium chloride
|
[Merged 2 duplicates: 1.6, 1.0] |
| 6 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
1 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 7 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
1 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 8 |
Fe SO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 |
H2SO4
H2O4S 98.079 g/mol
|
0.1 G_PER_L |
CHEBI:26836
sulfuric acid
|
Preparation
- 2:1 mixture
- See separate recipes. Autoclave separately. Mix aseptically when cool.
- Bring PJ (Mail sol. C 76) to the boil then add rye grass. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.
- Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.
- Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rpl_mp (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.753297Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C83 |
| 2026-03-13T03:09:14.048418Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:18:54.937387Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000397 |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.949717+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RPL:MP" to "rpl_mp" for consistent matching |
| 2026-06-05T07:41:44.694464+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.090447+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.875984+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7028b11f839200a1f7970d33ffce2498ba832bf0c35ce69484c9282b955c4cd5 |