Overview
- External ontology
- mediadive.medium:C88 (source link unavailable) — R75S:NSW
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'r75s', 'source': 'MediaDive', 'source_id': 'mediadive.medium:C87', 'original_category': 'bacterial'}
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_R75S_NSW.pdf
Composition
flowchart LR
CultureMech_000402["r75s_nsw"]
ing0_Natural_sea_water["Natural sea water"]
CultureMech_000402 --> ing0_Natural_sea_water
ing1_Rye_grass["Rye grass"]
CultureMech_000402 --> ing1_Rye_grass
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Natural sea water | 750 G_PER_L | ||
| 2 | Rye grass | 1 G_PER_L |
Preparation
- 2:1 mixture
- See separate recipes below. Autoclave separately. Mix aseptically when cool.
- Bring 75% seawater to the boil, add the powdered rye grass and keep boiling for 5 minutes. Allow to cool and restore volume to 1 litre with deionized water. Filter through Whatman GF/C paper. Autoclave at 15 psi for 15 minutes.
- Filter natural seawater through GF/C filters and autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- r75s (normalized source unresolved)
- r75s_nsw (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.768126Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C88 |
| 2026-03-13T03:09:16.070067Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:54.959618Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000402 |
| 2026-03-17T19:45:05.972220+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R75S:NSW" to "r75s_nsw" for consistent matching |
| 2026-08-06T03:05:54.193616+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: r75s, r75s_nsw |
| 2026-08-06T03:05:54.193654+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint b8064bbd75a4b99db4673d356f8e237c19119c8d5e5baa4f2a249b38e209ed6b |