sbbm

Overview

External ontology

mediadive.medium:C89 (source link unavailable) — SBBM

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SBBM.pdf

Composition

flowchart LR
    CultureMech_000403["sbbm"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000403 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000403 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000403 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000403 --> ing3_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000403 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NaCl["NaCl"]
    CultureMech_000403 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_000403 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_000403 --> ing7_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing7_Thiamine_hydrochloride -.-> CHEBI_49105
    ing8_Na2_EDTA["Na2-EDTA"]
    CultureMech_000403 --> ing8_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing8_Na2_EDTA -.-> CHEBI_64734
    ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000403 --> ing9_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000403 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_ZnCl2["ZnCl2"]
    CultureMech_000403 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000403 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_000403 --> ing13_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000403 --> ing14_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing14_Cyanocobalamine -.-> CHEBI_17439
    ing15_Soil["Soil"]
    CultureMech_000403 --> ing15_Soil
    ing16_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000403 --> ing16_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing16_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing17_NaHCO3["NaHCO3"]
    CultureMech_000403 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000403 --> ing18_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing18_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing19_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_000403 --> ing19_FeNa_EDTA
    ing20_H3BO3["H3BO3"]
    CultureMech_000403 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000403 --> ing21__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing21__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing22_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000403 --> ing22_Thiamine_HCl
    ing22_Thiamine_HCl -.-> CHEBI_49105
    ing23_Biotine["Biotine"]
    CultureMech_000403 --> ing23_Biotine
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotine -.-> CHEBI_15956

Ingredients (24)

Preparation

1. Used for some filamentous green algae e.g. Trentepohlia, can give better growth than 3N-BBM+V (Mail sol. C 14) or JM:SE (Mail sol. C 41). Make both media and sterilise, then mix aseptically in the ratio of 97% 3N-BBM+V and 3% SE2.
2. Once elements are dissolved autoclave at 15 psi for 15 minutes.
3. Make up to 1 litre with distilled water. For agar add 15 g per litre Bacterialogical Agar. Autoclave at 15 psi for 15 minutes.
4. Filter sterile
5. Ensure elements are added in the following sequence:
6. Once elements are dissolved autoclave at 15 psi for 15 minutes.
7. Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.
8. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
9. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2­4 mm) or stored as it is prior to use.
10. Soil is prepared as above. 105 g of air­dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes, then again after 24 hours. The contents of the bottle are left to settle (usually for at least a week) and then the supernatant is decanted and filtered. The final pH should be 7.0 - 8.0.
11. This recipe is a 7:3 mixture of JM and SE2. Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
12. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
13. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
14. Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• sbbm (normalized source unresolved)