Overview
- External ontology
- mediadive.medium:C8 (source link unavailable) — ASW 300 + barley
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'asw_150_barley', 'source': 'MediaDive', 'source_id': 'mediadive.medium:C6', 'original_category': 'bacterial'}
- {'name': 'asw_225_barley', 'source': 'MediaDive', 'source_id': 'mediadive.medium:C7', 'original_category': 'bacterial'}
- High-metal flag
- True
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_ASW_300.pdf
Composition
flowchart LR
CultureMech_000404["asw_300_barley"]
ing0_NaCl["NaCl"]
CultureMech_000404 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_000404 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000404 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000404 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000404 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
136 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
3.8 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
8.9 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.9 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.65 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Add 20 ml of the autoclaved artificial seawater with 300 psu and 4 autoclaved barley grains to a T25 tissue culture flask (Corning 25100).
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- asw_150_barley (normalized source unresolved)
- asw_225_barley (normalized source unresolved)
- asw_300_barley (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.492666Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C8 |
| 2026-03-13T06:18:54.980268Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000404 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.992533+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ASW 300 + barley" to "asw_300_barley" for consistent matching |
| 2026-06-05T07:40:49.948249+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:24.315449+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: asw_150_barley, asw_225_barley, asw_300_barley |
| 2026-08-06T03:05:24.315501+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 009bee0b2b1783ee163c85cf426c36e95300e7b7f784e4ad192b2ca578b134cd |