roufs_medium

CultureMech:000440 bacterial COMPLEX SOLID_AGAR
Original name: ROUF'S MEDIUM

Overview

External ontology
mediadive.medium:1019 (source link unavailable) — ROUF'S MEDIUM
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1019.pdf

Composition

flowchart LR
    CultureMech_000440["roufs_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000440 --> ing0_Yeast_extract
    ing1_Peptone["Peptone"]
    CultureMech_000440 --> ing1_Peptone
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000440 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_000440 --> ing3_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing3_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing4_Fe_NH4_citrate["Fe(NH4)citrate"]
    CultureMech_000440 --> ing4_Fe_NH4_citrate
    CHEBI_31604["CHEBI:31604"]
    ing4_Fe_NH4_citrate -.-> CHEBI_31604
    ing5_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_000440 --> ing5_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing5_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing6_FeCl3_x_4_H2O["FeCl3 x 4 H2O"]
    CultureMech_000440 --> ing6_FeCl3_x_4_H2O
    CHEBI_30808["CHEBI:30808"]
    ing6_FeCl3_x_4_H2O -.-> CHEBI_30808
    ing7_Agar["Agar"]
    CultureMech_000440 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000440 --> ing8_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000440 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000440 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000440 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000440 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_NaCl["NaCl"]
    CultureMech_000440 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000440 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_Biotin["Biotin"]
    CultureMech_000440 --> ing15_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_Biotin -.-> CHEBI_15956
    ing16_Folic_acid["Folic acid"]
    CultureMech_000440 --> ing16_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing16_Folic_acid -.-> CHEBI_27470
    ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000440 --> ing17_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing18_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000440 --> ing18_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing18_Thiamine_HCl -.-> CHEBI_49105
    ing19_Riboflavin["Riboflavin"]
    CultureMech_000440 --> ing19_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing19_Riboflavin -.-> CHEBI_17015
    ing20_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000440 --> ing20_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing20_Nicotinic_acid -.-> CHEBI_15940
    ing21_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000440 --> ing21_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing21_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing22_Vitamin_B12["Vitamin B12"]
    CultureMech_000440 --> ing22_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing22_Vitamin_B12 -.-> CHEBI_176843
    ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000440 --> ing23_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing24__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000440 --> ing24__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing24__DL__alpha_Lipoic_acid -.-> CHEBI_16494

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L
2 Peptone 5 G_PER_L
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.05 G_PER_L CHEBI:91243
calcium chloride hexahydrate
5 Fe(NH4)citrate 0.015 G_PER_L CHEBI:31604
ferric ammonium citrate
6 MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
0.05 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
7 FeCl3 x 4 H2O
Cl3Fe 162.204 g/mol
0.01 G_PER_L CHEBI:30808
iron trichloride
8 Agar 17 G_PER_L CHEBI:2509
agar
 (for solid medium)
9 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.17 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 NaCl
Cl.Na 58.443 g/mol
0.1 G_PER_L CHEBI:26710
sodium chloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
17 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
18 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
19 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
20 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
21 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
22 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
23 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
24 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
25 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. pH 7.1 (adjusted with NaOH if needed)
  2. The medium may be solifidied with 17 g/l agar.
  3. First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:13.123047Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1019
2026-02-06T05:10:33.022191Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:55.295727Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000440
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.342087+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ROUF'S MEDIUM" to "roufs_medium" for consistent matching
2026-06-05T07:41:44.657679+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:54.824135+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4b99f6950ffcf3eeb8e29c9281bf3d2a4bed3e7acd95efec342a83e8a65e13b3