Overview
- External ontology
- mediadive.medium:1020 (source link unavailable) — SULFURICURVUM (MBM) MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'mbm_medium_modified', 'source': 'KOMODO', 'source_id': 'komodo.medium:1020', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1020.pdf
Composition
flowchart LR
CultureMech_000442["sulfuricurvum_mbm_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_000442 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_KH2PO4["KH2PO4"]
CultureMech_000442 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_000442 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000442 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_000442 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000442 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_000442 --> ing6_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing6_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing7_Na2_EDTA["Na2-EDTA"]
CultureMech_000442 --> ing7_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing7_Na2_EDTA -.-> CHEBI_64734
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000442 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000442 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000442 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_000442 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000442 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000442 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000442 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000442 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
0.199601 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.199601 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.199601 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.399202 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.199601 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0998004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.49501 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 8 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Dissolve ingredients, except sodium thiosulfate, then sparge solution with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 7.0, then distribute medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml medium in 100 ml bottles) and autoclave. Prior to inoculation, add thiosulfate from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.
- After inoculation, pressurize culture vessels to 0.5 bar overpressure with sterile 100% H2 gas.
- First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- mbm_medium_modified (CultureMech:003532)
- sulfuricurvum_mbm_medium (CultureMech:000442)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.126619Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1020 |
| 2026-03-13T06:18:55.307798Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000442 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.356420+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFURICURVUM (MBM) MEDIUM" to "sulfuricurvum_mbm_medium" for consistent matching |
| 2026-06-05T07:41:47.636590+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.095864+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.331878+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: mbm_medium_modified, sulfuricurvum_mbm_medium |
| 2026-08-06T03:05:42.331924+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 4393eaf9b1726a8511a3e974eda746707d004190f8bf4d184a911bf9158e9312 |