nitratiruptor_and_nitratifractor_medium

CultureMech:000446 bacterial DEFINED LIQUID
Original name: NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM

Overview

External ontology
mediadive.medium:1024 (source link unavailable) — NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'nitratiruptor_and_nitratifactor_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1024', 'original_category': 'bacterial'}
pH
7.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1024.pdf

Composition

flowchart LR
    CultureMech_000446["nitratiruptor_and_nitratifractor_medium"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000446 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_NaHCO3["NaHCO3"]
    CultureMech_000446 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000446 --> ing2_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing2_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing3_Sulfur_powder["Sulfur powder"]
    CultureMech_000446 --> ing3_Sulfur_powder
    CHEBI_33403["CHEBI:33403"]
    ing3_Sulfur_powder -.-> CHEBI_33403
    ing4_Biotin["Biotin"]
    CultureMech_000446 --> ing4_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing4_Biotin -.-> CHEBI_15956
    ing5_Folic_acid["Folic acid"]
    CultureMech_000446 --> ing5_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing5_Folic_acid -.-> CHEBI_27470
    ing6_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000446 --> ing6_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing6_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing7_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000446 --> ing7_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing7_Thiamine_HCl -.-> CHEBI_49105
    ing8_Riboflavin["Riboflavin"]
    CultureMech_000446 --> ing8_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing8_Riboflavin -.-> CHEBI_17015
    ing9_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000446 --> ing9_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing9_Nicotinic_acid -.-> CHEBI_15940
    ing10_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000446 --> ing10_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing10_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing11_Vitamin_B12["Vitamin B12"]
    CultureMech_000446 --> ing11_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing11_Vitamin_B12 -.-> CHEBI_176843
    ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000446 --> ing12_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing13__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000446 --> ing13__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing13__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing14_NaCl["NaCl"]
    CultureMech_000446 --> ing14_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing14_NaCl -.-> CHEBI_26710
    ing15_K2HPO4["K2HPO4"]
    CultureMech_000446 --> ing15_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing15_K2HPO4 -.-> CHEBI_131527
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000446 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17_NH4Cl["NH4Cl"]
    CultureMech_000446 --> ing17_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing17_NH4Cl -.-> CHEBI_31206
    ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000446 --> ing18_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing19_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000446 --> ing19_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing19_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing20_KCl["KCl"]
    CultureMech_000446 --> ing20_KCl
    CHEBI_32588["CHEBI:32588"]
    ing20_KCl -.-> CHEBI_32588
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000446 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000446 --> ing22_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing23_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_000446 --> ing23_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing23_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing24_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000446 --> ing24_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing24_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing25_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
    CultureMech_000446 --> ing25_MnSO4_x_2_H2O
    CHEBI_86356["CHEBI:86356"]
    ing25_MnSO4_x_2_H2O -.-> CHEBI_86356
    ing26_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000446 --> ing26_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing26_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing27_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000446 --> ing27_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing27_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing28_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000446 --> ing28_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing28_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing29_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000446 --> ing29_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing29_CuSO4_x_5_H2O -.-> CHEBI_31440
    CultureMech_000446 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
2 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
4 Sulfur powder 3 G_PER_L CHEBI:33403
elemental sulfur
5 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
6 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
7 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
8 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
9 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
10 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
11 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
12 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
13 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
14 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
15 NaCl
Cl.Na 58.443 g/mol
3001.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 3000.0, 1.0]
16 K2HPO4
HO4P.2K 174.174 g/mol
14 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
14.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 14.0, 0.1]
18 NH4Cl
Cl.H4N 53.492 g/mol
25 G_PER_L CHEBI:31206
ammonium chloride
19 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
343.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 340.0, 3.0]
20 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
418 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
21 KCl
Cl.K 74.551 g/mol
33 G_PER_L CHEBI:32588
potassium chloride
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.07500000000000001 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.05, 0.025]
23 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.050300000000000004 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 [Merged 2 duplicates: 0.05, 0.0003]
24 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
1 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
25 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
26 MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
0.5 G_PER_L CHEBI:86356
manganese(II) sulfate dihydrate
27 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
28 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
29 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
30 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
31 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
32 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
33 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding the vitamins and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, the NaHCO3 and vitamin solution.
  2. Increase the 80% H2 + 20% CO2 gas phase pressure to 300 kPa. The final pH is 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:13.138222Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1024
2026-01-28T19:48:15.488941Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:22.509772Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:54.104283Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:18:55.352818Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000446
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.400002+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM" to "nitratiruptor_and_nitratifractor_medium" for consistent matching
2026-06-05T07:41:39.717510+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.562607+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:51.381544+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: nitratiruptor_and_nitratifactor_medium, nitratiruptor_and_nitratifractor_medium
2026-08-06T03:05:51.381597+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 2fd0679222ad4a73e71ff470fd60328fd4b2e3fc5bcfaeeacbe2a4b9c282c75e