Overview
- External ontology
- mediadive.medium:104d (source link unavailable) — MALEDIVIBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.3, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104d.pdf
Composition
flowchart LR
CultureMech_000477["maledivibacter_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_000477 --> ing0_Trypticase_peptone
ing1_Meat_peptone["Meat peptone"]
CultureMech_000477 --> ing1_Meat_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_000477 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_000477 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000477 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_000477 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_Betaine_x_H2O["Betaine x H2O"]
CultureMech_000477 --> ing6_Betaine_x_H2O
CHEBI_17750["CHEBI:17750"]
ing6_Betaine_x_H2O -.-> CHEBI_17750
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000477 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000477 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_K2HPO4["K2HPO4"]
CultureMech_000477 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_KH2PO4["KH2PO4"]
CultureMech_000477 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_NaHCO3["NaHCO3"]
CultureMech_000477 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 5 G_PER_L | ||
| 2 | Meat peptone | 5 G_PER_L | ||
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
62.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 60.0, 2.0] |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
5.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 5.0, 0.5] |
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Betaine x H2O
C5H11NO2 117.148 g/mol
|
6 G_PER_L |
CHEBI:17750
glycine betaine
|
|
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Preparation
- Dissolve ingredients (except betaine and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Adjust the pH to 7.2. Thereafter, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add betaine and cysteine from sterile, anoxic stock solutions prepared under 100% N2. Adjust the pH of the complete medium to 7.3 - 7.5.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- maledivibacter_medium (CultureMech:000477)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.177973Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 104d |
| 2026-03-13T03:08:38.347984Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:18:55.646138Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000477 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.653622+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MALEDIVIBACTER MEDIUM" to "maledivibacter_medium" for consistent matching |
| 2026-05-20T06:01:25.284313+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5} |
| 2026-06-05T07:41:12.617256+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.826414+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:41.729878+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b3a23a22752b202bc02e8668ec02002978cd613239de6641f7f2643d956de589 |