Overview
- External ontology
- mediadive.medium:1063 (source link unavailable) — MICROCELLA ALKALIPHILA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1063.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_000496["microcella_alkaliphila_medium"]
ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_000496 --> ing0_Pancreatic_digest_of_casein
ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_000496 --> ing1_Peptic_digest_of_soybean_meal
ing2_Glucose["Glucose"]
CultureMech_000496 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_000496 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_000496 --> ing4_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Dipotassium_phosphate -.-> CHEBI_131527
ing5_Agar["Agar"]
CultureMech_000496 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Yeast_extract["Yeast extract"]
CultureMech_000496 --> ing6_Yeast_extract
ing7_KH2PO4["KH2PO4"]
CultureMech_000496 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000496 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_NaCl["NaCl"]
CultureMech_000496 --> ing9_NaCl
ing9_NaCl -.-> CHEBI_26710
ing10_NH4Cl["NH4Cl"]
CultureMech_000496 --> ing10_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing10_NH4Cl -.-> CHEBI_31206
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000496 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_HCl["HCl"]
CultureMech_000496 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000496 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_000496 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000496 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_H3BO3["H3BO3"]
CultureMech_000496 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000496 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000496 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000496 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000496 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_000496 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000496 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_D_____biotin["D-(+)-biotin"]
CultureMech_000496 --> ing23_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing23_D_____biotin -.-> CHEBI_15956
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_000496 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000496 --> ing25_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_pantothenate -.-> CHEBI_31345
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000496 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000496 --> ing27_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing27_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing28_NaHCO3["NaHCO3"]
CultureMech_000496 --> ing28_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing28_NaHCO3 -.-> CHEBI_32139
ing29_Na2CO3_anhydrous["Na2CO3 anhydrous"]
CultureMech_000496 --> ing29_Na2CO3_anhydrous
CHEBI_29377["CHEBI:29377"]
ing29_Na2CO3_anhydrous -.-> CHEBI_29377
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 2 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 3 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 5 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 6 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 | Yeast extract | 3 G_PER_L | ||
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
10 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
6.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
8 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 11 |
NH4Cl
Cl.H4N 53.492 g/mol
|
8 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 13 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 24 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.2 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.3 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.2 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 29 |
NaHCO3
CHO3.Na 84.006 g/mol
|
42 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 30 |
Na2CO3 anhydrous
CO3.2Na 105.988 g/mol
|
53 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- After sterilization add sterile 1M Na-sesquicarbonate solution to achieve the pH of 9.5 (see medium 31).
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- microcella_alkaliphila_medium (CultureMech:000496)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.276008Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1063 |
| 2026-03-13T06:18:55.853819Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000496 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.872597+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MICROCELLA ALKALIPHILA MEDIUM" to "microcella_alkaliphila_medium" for consistent matching |
| 2026-06-05T07:41:33.820670+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.809098+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:45.377112+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b71dfbd6f6e6057151d27abf73060e4103bd0ad862464792a88775f7d3166749 |