Overview
- External ontology
- mediadive.medium:1072 (source link unavailable) — PSEUDOALTEROMONAS SPIRALIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1072.pdf
Composition
flowchart LR
CultureMech_000504["pseudoalteromonas_spiralis_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000504 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000504 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NH4Cl["NH4Cl"]
CultureMech_000504 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KCl["KCl"]
CultureMech_000504 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000504 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na_acetate["Na-acetate"]
CultureMech_000504 --> ing5_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Na_acetate -.-> CHEBI_32954
ing6_Yeast_extract["Yeast extract"]
CultureMech_000504 --> ing6_Yeast_extract
ing7_Casamino_acids["Casamino acids"]
CultureMech_000504 --> ing7_Casamino_acids
ing8_NaCl["NaCl"]
CultureMech_000504 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000504 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000504 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_Biotin["Biotin"]
CultureMech_000504 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000504 --> ing12_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing12_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing13_Nicotinic_acid["Nicotinic acid"]
CultureMech_000504 --> ing13_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing13_Nicotinic_acid -.-> CHEBI_15940
ing14_Vitamin_B12["Vitamin B12"]
CultureMech_000504 --> ing14_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing14_Vitamin_B12 -.-> CHEBI_176843
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 | Yeast extract | 0.5 G_PER_L | ||
| 8 | Casamino acids | 0.5 G_PER_L | ||
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.3 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.003 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.08 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.4 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 14 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.4 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 15 | Vitamin B12 | 0.02 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Adjust to pH 5.9 before autoklaving, adjust with NaHCO3 to pH 7.8 after autoklaving.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pseudoalteromonas_spiralis_medium (CultureMech:000504)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.293603Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1072 |
| 2026-01-28T19:48:37.157906Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:55.904597Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000504 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.927830+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PSEUDOALTEROMONAS SPIRALIS MEDIUM" to "pseudoalteromonas_spiralis_medium" for consistent matching |
| 2026-06-05T07:41:42.636542+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.845583+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:10.437007+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.616023+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c1a2f91f60caab98e1b076d45ce3ba135532232ee0b85fcfdc13d6983c8ad605 |