Overview
- External ontology
- mediadive.medium:1076a (source link unavailable) — SP4 MEDIUM WITH GLUCOSE
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1076a.pdf
Composition
flowchart LR
CultureMech_000509["sp4_medium_with_glucose"]
ing0_Glucose["Glucose"]
CultureMech_000509 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Tryptone["Tryptone"]
CultureMech_000509 --> ing1_Tryptone
ing2_Peptone["Peptone"]
CultureMech_000509 --> ing2_Peptone
ing3_PPLO_broth["PPLO broth"]
CultureMech_000509 --> ing3_PPLO_broth
ing4_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_000509 --> ing4_Fetal_bovine_serum
ing5_CMRL_1066["CMRL 1066"]
CultureMech_000509 --> ing5_CMRL_1066
ing6_Yeast_extract["Yeast extract"]
CultureMech_000509 --> ing6_Yeast_extract
ing7_Phenol_red["Phenol red"]
CultureMech_000509 --> ing7_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing7_Phenol_red -.-> CHEBI_31991
ing8_L_Glutamine["L-Glutamine"]
CultureMech_000509 --> ing8_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing8_L_Glutamine -.-> CHEBI_18050
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
0.52 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Tryptone | 9.70874 G_PER_L | ||
| 3 | Peptone | 5.14563 G_PER_L | ||
| 4 | PPLO broth | 3.39806 G_PER_L | ||
| 5 | Fetal bovine serum | 90 G_PER_L | ||
| 6 | CMRL 1066 | 25 G_PER_L | ||
| 7 | Yeast extract | 4.5 G_PER_L | [Merged 2 duplicates: 3.5, 1.0] | |
| 8 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.01 G_PER_L |
CHEBI:31991
phenol red
|
|
| 9 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
0.0496876 G_PER_L |
CHEBI:18050
L-glutamine
|
Preparation
- Use medium 1076, but add 5.2 ml 10% glucose (filter-sterilized).
- Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
- Add aseptically:
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sp4_medium_with_glucose (CultureMech:000509)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.307214Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1076a |
| 2026-03-13T03:08:50.355711Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:55.946406Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000509 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.972416+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP4 MEDIUM WITH GLUCOSE" to "sp4_medium_with_glucose" for consistent matching |
| 2026-06-05T07:41:46.124463+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:55.964748+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9836e27474fd71d30eac00be20dbe19834486f5995a418bc87ee0c4e73fde6e2 |