Overview
- External ontology
- mediadive.medium:1076b (source link unavailable) — SP4-Z MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1076b.pdf
Composition
flowchart LR
CultureMech_000510["sp4_z_medium"]
ing0_PPLO_broth["PPLO broth"]
CultureMech_000510 --> ing0_PPLO_broth
ing1_Tryptone["Tryptone"]
CultureMech_000510 --> ing1_Tryptone
ing2_Bacto_peptone["Bacto peptone"]
CultureMech_000510 --> ing2_Bacto_peptone
ing3_DNA["DNA"]
CultureMech_000510 --> ing3_DNA
CHEBI_16991["CHEBI:16991"]
ing3_DNA -.-> CHEBI_16991
ing4_CMRL_1066["CMRL 1066"]
CultureMech_000510 --> ing4_CMRL_1066
ing5_Yeast_extract["Yeast extract"]
CultureMech_000510 --> ing5_Yeast_extract
ing6_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_000510 --> ing6_Fetal_bovine_serum
ing7_Swine_serum["Swine serum"]
CultureMech_000510 --> ing7_Swine_serum
ing8_Glucose["Glucose"]
CultureMech_000510 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Urea["Urea"]
CultureMech_000510 --> ing9_Urea
CHEBI_16199["CHEBI:16199"]
ing9_Urea -.-> CHEBI_16199
ing10_Agar["Agar"]
CultureMech_000510 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | PPLO broth | 3.5 G_PER_L | ||
| 2 | Tryptone | 10 G_PER_L | ||
| 3 | Bacto peptone | 5 G_PER_L | ||
| 4 | DNA | 0.2 G_PER_L |
CHEBI:16991
deoxyribonucleic acid
|
|
| 5 | CMRL 1066 | 10 G_PER_L | ||
| 6 | Yeast extract | 0.4 G_PER_L | ||
| 7 | Fetal bovine serum | 17 G_PER_L | ||
| 8 | Swine serum | 17 G_PER_L | ||
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 |
Urea
CH4N2O 60.056 g/mol
|
2 G_PER_L |
CHEBI:16199
urea
|
|
| 11 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- The difference between this medium and "normal" SP4 medium (Medium 1076) is that the yeast extract was left out and DNA was added instead. Since most mycoplasmas use either glucose or arginine as their energy source one is added to the media. Do not combine both in one medium, arginine can inhibit the growth of some glucose utilizers (unknown reason).
- Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
- Add aseptically:
- As energy source add either:
- For Ureaplasma add instead of Glucose or Arginine:
- For agar plates use only 126 ml of distilled water and add 2 g of agar. Medium containing agar should be placed at 55°C before adding supplements.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sp4_z_medium (CultureMech:000510)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.308987Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1076b |
| 2026-03-13T06:18:55.952135Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000510 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.978012+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP4-Z MEDIUM" to "sp4_z_medium" for consistent matching |
| 2026-06-05T07:41:46.130600+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:55.972558+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint eae011848eef34bf43c499cad6247c73bb63c8f925d36da902f481884b166132 |