Overview
- External ontology
- mediadive.medium:1081a (source link unavailable) — HORIKOSHI-1 MEDIUM WITH 10% NaCl
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1081a.pdf
Composition
flowchart LR
CultureMech_000516["horikoshi_1_medium_with_10_nacl"]
ing0_NaCl["NaCl"]
CultureMech_000516 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Glucose["Glucose"]
CultureMech_000516 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Polypeptone["Polypeptone"]
CultureMech_000516 --> ing2_Polypeptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_000516 --> ing3_Yeast_extract
ing4_K2HPO4["K2HPO4"]
CultureMech_000516 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000516 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Agar["Agar"]
CultureMech_000516 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Na2CO3["Na2CO3"]
CultureMech_000516 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 | Polypeptone | 5 G_PER_L | ||
| 4 | Yeast extract | 5 G_PER_L | ||
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- Use medium 1081 (HORIKOSHI-1 MEDIUM), but add 100 g NaCl.
- After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- horikoshi_1_medium_with_10_nacl (CultureMech:000516)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.319544Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1081a |
| 2026-03-13T06:18:55.980537Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000516 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.007209+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HORIKOSHI-1 MEDIUM WITH 10% NaCl" to "horikoshi_1_medium_with_10_nacl" for consistent matching |
| 2026-06-05T07:41:08.691332+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.811383+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.785527+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 417466fd47b571a6962777c90c281de42591e90c6396f2db477fdf1d8b2b954b |