Overview
- External ontology
- mediadive.medium:1094 (source link unavailable) — METHANOSPHAERULA (PEAT) MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'methanosphaerula_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1094', 'original_category': 'archaea'}
- pH
- 5.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1094.pdf
Composition
flowchart LR
CultureMech_000525["methanosphaerula_peat_medium"]
ing0_KCl["KCl"]
CultureMech_000525 --> ing0_KCl
CHEBI_32588["CHEBI:32588"]
ing0_KCl -.-> CHEBI_32588
ing1_KH2PO4["KH2PO4"]
CultureMech_000525 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_000525 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_000525 --> ing3_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing3_Tris_HCl_buffer -.-> CHEBI_9754
ing4_Nitrilotriacetate["Nitrilotriacetate"]
CultureMech_000525 --> ing4_Nitrilotriacetate
CHEBI_25548["CHEBI:25548"]
ing4_Nitrilotriacetate -.-> CHEBI_25548
ing5_TiCl3["TiCl3"]
CultureMech_000525 --> ing5_TiCl3
ing6_2__N_Morpholino_ethanesulfonic_acid["2-(N-Morpholino)ethanesulfonic acid"]
CultureMech_000525 --> ing6_2__N_Morpholino_ethanesulfonic_acid
CHEBI_39005["CHEBI:39005"]
ing6_2__N_Morpholino_ethanesulfonic_acid -.-> CHEBI_39005
ing7_NaOH["NaOH"]
CultureMech_000525 --> ing7_NaOH
CHEBI_32145["CHEBI:32145"]
ing7_NaOH -.-> CHEBI_32145
ing8_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
CultureMech_000525 --> ing8_2_Mercaptoethanesulfonate
CHEBI_17905["CHEBI:17905"]
ing8_2_Mercaptoethanesulfonate -.-> CHEBI_17905
ing9_Na_acetate["Na-acetate"]
CultureMech_000525 --> ing9_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Na_acetate -.-> CHEBI_32954
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000525 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Na2_EDTA["Na2-EDTA"]
CultureMech_000525 --> ing11_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing11_Na2_EDTA -.-> CHEBI_64734
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000525 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000525 --> ing13_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing13_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000525 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000525 --> ing15_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000525 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_ZnCl2["ZnCl2"]
CultureMech_000525 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_H3BO3["H3BO3"]
CultureMech_000525 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000525 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2Mo4_x_2_H2O["Na2Mo4 x 2 H2O"]
CultureMech_000525 --> ing20_Na2Mo4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2Mo4_x_2_H2O -.-> CHEBI_75213
ing21_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_000525 --> ing21_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing21_MnSO4_x_4_H2O -.-> CHEBI_86358
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000525 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_Biotin["Biotin"]
CultureMech_000525 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_000525 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000525 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_000525 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_000525 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_000525 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_000525 --> ing29_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
CultureMech_000525 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KCl
Cl.K 74.551 g/mol
|
0.00159067 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0144221 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.0284199 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
7.2 G_PER_L |
CHEBI:9754
tris
|
|
| 5 |
Nitrilotriacetate
C6H6NO6 188.115 g/mol
|
0.458736 G_PER_L |
CHEBI:25548
nitrilotriacetate(3-)
|
|
| 6 | TiCl3 | 6.34615 G_PER_L | ||
| 7 |
2-(N-Morpholino)ethanesulfonic acid
C6H13NO4S 195.24 g/mol
|
97.5 G_PER_L |
CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
|
|
| 8 |
NaOH
HO.Na 39.997 g/mol
|
10 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 9 |
2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
|
8 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 10 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
41 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.96 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
37.23 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.34 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
3.45 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 16 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.56 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.024 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.075 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.019 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2Mo4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.026 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.009 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Solutions B, C, D and G are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions E and F are prepared under 100% N2 gas atmosphere and autoclaved. To complete the medium add appropriate amounts of solutions B to G to the sterile solution A in the sequence indicated and let medium equilibrate overnight. Adjust pH of complete medium to 5.7, if necessary.
- After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- Note: The complete medium is not stable and can be stored only for a short time.
- Adjust to pH 7.5 with NaOH.
- Dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- methanosphaerula_medium (CultureMech:003744)
- methanosphaerula_peat_medium (CultureMech:000525)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.347963Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1094 |
| 2026-02-06T05:10:19.465523Z | invalid-chebi-removal | Removed 2 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:18:56.065042Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000525 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.099104+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOSPHAERULA (PEAT) MEDIUM" to "methanosphaerula_peat_medium" for consistent matching |
| 2026-06-05T07:41:32.699651+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.404157+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:31.405716+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: methanosphaerula_medium, methanosphaerula_peat_medium |
| 2026-08-06T03:04:31.405777+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 1238714ddfd603afe03fe4dadf773ecf06020ad6d2fc2f258a52cc933bc02f00 |