Overview
- External ontology
- mediadive.medium:1122a (source link unavailable) — METHYLOVERSATILIS THERMOTOLERANS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_k', 'source': 'KOMODO', 'source_id': 'komodo.medium:1122', 'original_category': 'bacterial'}
- {'name': 'for_dsm_23053', 'source': 'KOMODO', 'source_id': 'komodo.medium:1122.1', 'original_category': 'bacterial'}
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1122a.pdf
Composition
flowchart LR
CultureMech_000560["methyloversatilis_thermotolerans_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000560 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_000560 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaCl["NaCl"]
CultureMech_000560 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000560 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000560 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_Agar["Agar"]
CultureMech_000560 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Methanol["Methanol"]
CultureMech_000560 --> ing6_Methanol
CHEBI_17790["CHEBI:17790"]
ing6_Methanol -.-> CHEBI_17790
ing7_Vitamin_B12["Vitamin B12"]
CultureMech_000560 --> ing7_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing7_Vitamin_B12 -.-> CHEBI_176843
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.025 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.002 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
Methanol
CH4O 32.042 g/mol
|
0.5 G_PER_L |
CHEBI:17790
methanol
|
|
| 8 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Adjust to pH 7.2.
- After autoclaving add methanol (sterilized separately) to a concentration of 0.5 % (v/v) and 0.1 ml vitamin solution B12 (10µg /ml, filter sterilized).
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- KOMODO_1122_MEDIUM_K (CultureMech:003826)
- for_dsm_23053 (CultureMech:003825)
- methyloversatilis_thermotolerans_medium (CultureMech:000560)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.449698Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1122a |
| 2026-03-13T06:18:56.392588Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000560 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.471726+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOVERSATILIS THERMOTOLERANS MEDIUM" to "methyloversatilis_thermotolerans_medium" for consistent matching |
| 2026-06-05T07:41:33.541508+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.719104+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:40.287849+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: KOMODO_1122_MEDIUM_K, for_dsm_23053, methyloversatilis_thermotolerans_medium |
| 2026-08-06T03:04:40.287917+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 1393bb9a30567320e0cf1d53a58815aaf68b89b104ebed85e45a4f93eb22df94 |