Overview
- External ontology
- mediadive.medium:1150 (source link unavailable) — HALOALKALIPHILIC GROWTH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1150.pdf
Composition
flowchart LR
CultureMech_000589["haloalkaliphilic_growth_medium"]
ing0_Glucose["Glucose"]
CultureMech_000589 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_000589 --> ing1_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing1_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing2_NaNO3["NaNO3"]
CultureMech_000589 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_NH4Cl["NH4Cl"]
CultureMech_000589 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KH2PO4["KH2PO4"]
CultureMech_000589 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Yeast_extract["Yeast extract"]
CultureMech_000589 --> ing5_Yeast_extract
ing6_Casamino_acids["Casamino acids"]
CultureMech_000589 --> ing6_Casamino_acids
ing7_NaCl["NaCl"]
CultureMech_000589 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
4 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
0.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 | Yeast extract | 1 G_PER_L | ||
| 7 | Casamino acids | 0.5 G_PER_L | ||
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Adjust to pH 10.0 with concentrated NaOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- haloalkaliphilic_growth_medium (CultureMech:000589)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.519778Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1150 |
| 2026-01-28T19:48:37.050152Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:56.627892Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000589 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.717378+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOALKALIPHILIC GROWTH MEDIUM" to "haloalkaliphilic_growth_medium" for consistent matching |
| 2026-06-05T07:41:07.338518+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.573387+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:59.380666+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.087562+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint cf639565fdb77ae784d1399ccce03def254f35e69d9801fb0759e95b5470fbc5 |