pm_medium

CultureMech:000591 bacterial DEFINED SOLID_AGAR
Original name: PM MEDIUM

Overview

External ontology
mediadive.medium:1152 (source link unavailable) — PM MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1152.pdf

Composition

flowchart LR
    CultureMech_000591["pm_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000591 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_000591 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_NaCl["NaCl"]
    CultureMech_000591 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000591 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000591 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NH4Cl["NH4Cl"]
    CultureMech_000591 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_CaCl2["CaCl2"]
    CultureMech_000591 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000591 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_Na_acetate["Na acetate"]
    CultureMech_000591 --> ing8_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Na_acetate -.-> CHEBI_32954
    ing9_Agar["Agar"]
    CultureMech_000591 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_HCl["HCl"]
    CultureMech_000591 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000591 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_000591 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000591 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_000591 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000591 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000591 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000591 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000591 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 Na2HPO4
HO4P.2Na 141.958 g/mol
11.2 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 NaCl
Cl.Na 58.443 g/mol
4 G_PER_L CHEBI:26710
sodium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 NH4Cl
Cl.H4N 53.492 g/mol
2 G_PER_L CHEBI:31206
ammonium chloride
7 CaCl2
Ca.2Cl 110.984 g/mol
0.01 G_PER_L CHEBI:3312
calcium dichloride
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0 MICROG_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 Na acetate
C2H3O2.Na 82.034 g/mol
10 G_PER_L CHEBI:32954
sodium acetate
10 Agar 20 G_PER_L CHEBI:2509
agar
 (for solid medium)
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust to pH 7.2. The medium may be solidified by adding 20.0 g/l agar.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:13.525060Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1152
2026-03-13T06:18:56.647370Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000591
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.739477+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PM MEDIUM" to "pm_medium" for consistent matching
2026-06-05T07:41:42.294783+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.335248+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.322787+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint e25171f4b4e6351467857d2d58786dd812dc393cb2e5fca76e54253f67c7e089