Overview
- External ontology
- mediadive.medium:1155 (source link unavailable) — SME MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1155.pdf
Composition
flowchart LR
CultureMech_000594["sme_medium"]
ing0_NaCl["NaCl"]
CultureMech_000594 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000594 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000594 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_KH2PO4["KH2PO4"]
CultureMech_000594 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000594 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KCl["KCl"]
CultureMech_000594 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_000594 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_NaBr["NaBr"]
CultureMech_000594 --> ing7_NaBr
CHEBI_63004["CHEBI:63004"]
ing7_NaBr -.-> CHEBI_63004
ing8_H3BO3["H3BO3"]
CultureMech_000594 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_000594 --> ing9_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing9_SrCl2_x_6_H2O -.-> CHEBI_36385
ing10_KJ["KJ"]
CultureMech_000594 --> ing10_KJ
ing11_Elemental_sulfur["Elemental sulfur"]
CultureMech_000594 --> ing11_Elemental_sulfur
CHEBI_33403["CHEBI:33403"]
ing11_Elemental_sulfur -.-> CHEBI_33403
ing12_Double_distilled_water["Double distilled water"]
CultureMech_000594 --> ing12_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing12_Double_distilled_water -.-> CHEBI_15377
ing13_Na2S["Na2S"]
CultureMech_000594 --> ing13_Na2S
CHEBI_76208["CHEBI:76208"]
ing13_Na2S -.-> CHEBI_76208
ing14_Yeast_extract["Yeast extract"]
CultureMech_000594 --> ing14_Yeast_extract
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
27.7 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.75 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.66 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 8 |
NaBr
Br.Na 102.894 g/mol
|
0.1 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 10 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.015 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 11 | KJ | 50 G_PER_L | ||
| 12 | Elemental sulfur | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
|
| 13 |
Double distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
|
| 14 |
Na2S
2Na.S 78.047 g/mol
|
0.5 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
(used for reduction of the medium) |
| 15 | Yeast extract | 0.4 G_PER_L |
Preparation
- Reduction of the medium by addition of 20 ml Na2S (2.5%); then adjust pH to 5.5 with sulfuric acid.
- Gas phase: H2/CO2=80/20 (250 kPa).
- After autoclaving add 0.04% yeast extract.
- Incubation at 90°C for 1-2 days.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sme_medium (CultureMech:000594)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.532602Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1155 |
| 2026-03-13T06:18:56.669442Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000594 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.765215+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SME MEDIUM" to "sme_medium" for consistent matching |
| 2026-06-05T07:41:45.944868+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.525411+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.828425+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 337116e4b42059e5252b99e8869bd9565c1f6fba914eea7405b1d3607f97bb37 |