desulfuribacillus_medium_arsenate

CultureMech:000608 bacterial COMPLEX LIQUID
Original name: DESULFURIBACILLUS MEDIUM (ARSENATE)

Overview

External ontology
mediadive.medium:1166a (source link unavailable) — DESULFURIBACILLUS MEDIUM (ARSENATE)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'aso4_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1166', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_22071', 'source': 'KOMODO', 'source_id': 'komodo.medium:1166.1', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_22410', 'source': 'KOMODO', 'source_id': 'komodo.medium:1166.3', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_24176_and_dsm_24629', 'source': 'KOMODO', 'source_id': 'komodo.medium:1166.4', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_24179', 'source': 'KOMODO', 'source_id': 'komodo.medium:1166.5', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_24400', 'source': 'KOMODO', 'source_id': 'komodo.medium:1166.2', 'original_category': 'bacterial'}
pH range
{'min': 9.5, 'max': 10.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1166a.pdf

Composition

flowchart LR
    CultureMech_000608["desulfuribacillus_medium_arsenate"]
    ing0_NaCl["NaCl"]
    CultureMech_000608 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000608 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Na2SO4["Na2SO4"]
    CultureMech_000608 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_NaHCO3["NaHCO3"]
    CultureMech_000608 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Na2CO3["Na2CO3"]
    CultureMech_000608 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_NH4Cl["NH4Cl"]
    CultureMech_000608 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000608 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000608 --> ing7_Yeast_extract
    ing8_Na_pyruvate["Na-pyruvate"]
    CultureMech_000608 --> ing8_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing8_Na_pyruvate -.-> CHEBI_50144
    ing9_Na2HAsO4_x_7_H2O["Na2HAsO4 x 7 H2O"]
    CultureMech_000608 --> ing9_Na2HAsO4_x_7_H2O
    CHEBI_91257["CHEBI:91257"]
    ing9_Na2HAsO4_x_7_H2O -.-> CHEBI_91257
    ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000608 --> ing10_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_x_9_H2O -.-> CHEBI_76209
    ing11_EDTA["EDTA"]
    CultureMech_000608 --> ing11_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing11_EDTA -.-> CHEBI_4735
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000608 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000608 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000608 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_000608 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000608 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000608 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000608 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000608 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_NaOH["NaOH"]
    CultureMech_000608 --> ing20_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing20_NaOH -.-> CHEBI_32145
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000608 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000608 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing23_Biotin["Biotin"]
    CultureMech_000608 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_000608 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000608 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000608 --> ing26_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing26_Thiamine_HCl -.-> CHEBI_49105
    ing27_Riboflavin["Riboflavin"]
    CultureMech_000608 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000608 --> ing28_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing28_Nicotinic_acid -.-> CHEBI_15940
    ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000608 --> ing29_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
    CultureMech_000608 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
5.98205 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.997009 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 Na2SO4
2Na.O4S 142.043 g/mol
2.79163 G_PER_L CHEBI:32149
sodium sulfate
4 NaHCO3
CHO3.Na 84.006 g/mol
7.97607 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 Na2CO3
CO3.2Na 105.988 g/mol
21.9342 G_PER_L CHEBI:29377
sodium carbonate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.199402 G_PER_L CHEBI:31206
ammonium chloride
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.199402 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 Yeast extract 0.0498504 G_PER_L
9 Na-pyruvate
C3H3O3.Na 110.044 g/mol
2.19342 G_PER_L CHEBI:50144
sodium pyruvate
10 Na2HAsO4 x 7 H2O
7H2O.HAsO4.2Na 312.011 g/mol
6.18146 G_PER_L CHEBI:91257
disodium hydrogenarsenate heptahydrate
11 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.239282 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
12 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.03 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
25 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
26 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
27 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
29 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
30 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
31 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
32 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
33 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve sodium chloride, hydrogenphosphate and sodium sulfate, then sparge solution with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve hydrogencarbonate and carbonate, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, pyruvate, arsenate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of vitamins, pyruvate and arsenate should be sterilized by filtration. Adjust pH of complete medium to 9.5 - 10.
  2. pH 3.0-4.0

Target organisms

No target organisms are recorded.

Provenance

Merged from 7 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:13.570320Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1166a
2026-02-06T05:10:25.398243Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:56.784041Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000608
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:07.895324+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFURIBACILLUS MEDIUM (ARSENATE)" to "desulfuribacillus_medium_arsenate" for consistent matching
2026-05-20T06:01:15.156889+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '9.5-10' -> {'min': 9.5, 'max': 10.0}
2026-06-05T07:40:58.199218+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.186971+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:24.243982+00:00 recipe-merger Merged 7 duplicate recipes into canonical record Sources: aso4_medium, desulfuribacillus_medium_arsenate, for_dsm_22071, for_dsm_22410, for_dsm_24176_and_dsm_24629, for_dsm_24179, for_dsm_24400
2026-08-06T03:05:24.244107+00:00 merge_recipes.py MERGED_RECIPES merged 7 source recipe(s) on fingerprint d7603e51895bd6427df9577430e83dc0555a1bb5e40d4d473decfb07678c4ede