Overview
- External ontology
- mediadive.medium:1179 (source link unavailable) — NMS MEDIUM FOR METHYLOCYSTIS SP.
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'nms_medium_for_methanotrophs', 'source': 'KOMODO', 'source_id': 'komodo.medium:1179', 'original_category': 'bacterial'}
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1179.pdf
Composition
flowchart LR
CultureMech_000621["nms_medium_for_methylocystis_sp"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000621 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_000621 --> ing1_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing1_CaCl2_x_6_H2O -.-> CHEBI_91243
ing2_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_000621 --> ing2_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing2_Fe_III__EDTA -.-> CHEBI_30729
ing3_KNO3["KNO3"]
CultureMech_000621 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_KH2PO4["KH2PO4"]
CultureMech_000621 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_000621 --> ing5_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_000621 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000621 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000621 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000621 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_H3BO3["H3BO3"]
CultureMech_000621 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000621 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000621 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000621 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000621 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.2 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 3 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
0.004 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
|
| 4 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.272 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
0.717 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.01 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.003 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.02 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.001 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.002 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 6.8.
- Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 50% methane to the gas phase Autoclave at 121°C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- nms_medium_for_methanotrophs (CultureMech:003899)
- nms_medium_for_methylocystis_sp (CultureMech:000621)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.598572Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1179 |
| 2026-03-13T06:18:56.858409Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000621 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:07.986658+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NMS MEDIUM FOR METHYLOCYSTIS SP." to "nms_medium_for_methylocystis_sp" for consistent matching |
| 2026-06-05T07:41:40.028075+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.708255+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:09.666169+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.586551+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: nms_medium_for_methanotrophs, nms_medium_for_methylocystis_sp |
| 2026-08-06T03:05:51.586593+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint d15490a7354e6afef3a809d7674af6877bb3daffc6afb07509361fbebb4ac151 |