methanobrevibacter_medium

Overview

External ontology

mediadive.medium:119a (source link unavailable) — METHANOBREVIBACTER MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119a.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_000644["methanobrevibacter_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000644 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000644 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_000644 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000644 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000644 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000644 --> ing5_Yeast_extract
    ing6_Na_acetate["Na-acetate"]
    CultureMech_000644 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na_formate["Na-formate"]
    CultureMech_000644 --> ing7_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing7_Na_formate -.-> CHEBI_62965
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000644 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_000644 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000644 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000644 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_HCl["HCl"]
    CultureMech_000644 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000644 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing14_ZnCl2["ZnCl2"]
    CultureMech_000644 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000644 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_H3BO3["H3BO3"]
    CultureMech_000644 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000644 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000644 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000644 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000644 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Isobutyric_acid["Isobutyric acid"]
    CultureMech_000644 --> ing21_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing21_Isobutyric_acid -.-> CHEBI_16135
    ing22_Tryptone["Tryptone"]
    CultureMech_000644 --> ing22_Tryptone
    ing23_Yeast_extract["Yeast extract"]
    CultureMech_000644 --> ing23_Yeast_extract
    ing24_Sodium_chloride["Sodium chloride"]
    CultureMech_000644 --> ing24_Sodium_chloride
    ing24_Sodium_chloride -.-> CHEBI_26710
    ing25_Valeric_acid["Valeric acid"]
    CultureMech_000644 --> ing25_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing25_Valeric_acid -.-> CHEBI_17418
    ing26_Isovaleric_acid["Isovaleric acid"]
    CultureMech_000644 --> ing26_Isovaleric_acid
    CHEBI_28484["CHEBI:28484"]
    ing26_Isovaleric_acid -.-> CHEBI_28484
    ing27_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000644 --> ing27_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing27_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing28_H2SO4["H2SO4"]
    CultureMech_000644 --> ing28_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing28_H2SO4 -.-> CHEBI_26836

Ingredients (29)

Preparation

1. Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes to 30% of their volume and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
2. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure.
3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
4. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.
5. Adjust pH to 7.5 with concentrated NaOH.
6. The ferrous sulfate solution is not stable and should be freshly prepared.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• methanobrevibacter_medium (CultureMech:000644)