Overview
- External ontology
- mediadive.medium:11 (source link unavailable) — MRS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.2, 'max': 6.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium11.pdf
Composition
flowchart LR
CultureMech_000647["mrs_medium"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_000647 --> ing0_Casein_peptone
ing1_Meat_extract["Meat extract"]
CultureMech_000647 --> ing1_Meat_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_000647 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_000647 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Tween_80["Tween 80"]
CultureMech_000647 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_K2HPO4["K2HPO4"]
CultureMech_000647 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Na_acetate["Na-acetate"]
CultureMech_000647 --> ing6_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Na_acetate -.-> CHEBI_32954
ing7__NH4_3_citrate["(NH4)3 citrate"]
CultureMech_000647 --> ing7__NH4_3_citrate
CHEBI_63037["CHEBI:63037"]
ing7__NH4_3_citrate -.-> CHEBI_63037
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000647 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000647 --> ing9_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing9_MnSO4_x_H2O -.-> CHEBI_86364
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 10 G_PER_L | ||
| 2 | Meat extract | 10 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
(NH4)3 citrate
C6H5O7.3H4N 243.216 g/mol
|
2 G_PER_L |
CHEBI:63037
triammonium citrate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.05 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Preparation
- Adjust pH to 6.2 - 6.5.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mrs_medium (CultureMech:000647)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:09.971970Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 11 |
| 2026-03-13T06:18:57.063796Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000647 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.222970+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS MEDIUM" to "mrs_medium" for consistent matching |
| 2026-05-20T06:02:00.184889+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.2-6.5' -> {'min': 6.2, 'max': 6.5} |
| 2026-06-05T07:41:38.483052+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.191604+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.477860+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint abcd760ba289f3ca344a6053365af73787e83132905d3d6feadf2b9aec132838 |