Overview
- External ontology
- mediadive.medium:1204 (source link unavailable) — SPHAEROCHAETA MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'spirochaeta_coccoides_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1204', 'original_category': 'bacterial'}
- pH
- 7.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1204.pdf
Composition
flowchart LR
CultureMech_000655["sphaerochaeta_medium"]
ing0_NaCl["NaCl"]
CultureMech_000655 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_000655 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_NH4Cl["NH4Cl"]
CultureMech_000655 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KH2PO4["KH2PO4"]
CultureMech_000655 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Na2SO4["Na2SO4"]
CultureMech_000655 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000655 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000655 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Yeast_extract["Yeast extract"]
CultureMech_000655 --> ing7_Yeast_extract
ing8_Casein_peptone["Casein peptone"]
CultureMech_000655 --> ing8_Casein_peptone
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_000655 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Na2CO3["Na2CO3"]
CultureMech_000655 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_Maltose_monohydrate["Maltose monohydrate"]
CultureMech_000655 --> ing11_Maltose_monohydrate
ing12_DL_Dithiothreitol["DL-Dithiothreitol"]
CultureMech_000655 --> ing12_DL_Dithiothreitol
CHEBI_18320["CHEBI:18320"]
ing12_DL_Dithiothreitol -.-> CHEBI_18320
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.15 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 | Yeast extract | 4 G_PER_L | ||
| 9 | Casein peptone | 2 G_PER_L | ||
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 | Maltose monohydrate | 2 G_PER_L | ||
| 13 |
DL-Dithiothreitol
C4H10O2S2 154.256 g/mol
|
0.8 G_PER_L |
CHEBI:18320
1,4-dithiothreitol
|
Preparation
- Dissolve ingredients except carbonate, maltose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 minutes. Add maltose and DTT from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Check pH of complete medium and adjust to 7.4, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- sphaerochaeta_medium (CultureMech:000655)
- spirochaeta_coccoides_medium (CultureMech:003952)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.686026Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1204 |
| 2026-03-13T06:18:57.125147Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000655 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.292743+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPHAEROCHAETA MEDIUM" to "sphaerochaeta_medium" for consistent matching |
| 2026-06-05T07:41:46.150633+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.576274+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.000437+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: sphaerochaeta_medium, spirochaeta_coccoides_medium |
| 2026-08-06T03:05:56.000489+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ebb7ce9399d40a93f1d255be52811846fdf22329dad16572495a9573b4972adb |