methanosarcina_barkeri_medium

CultureMech:000661 archaea COMPLEX LIQUID
Original name: METHANOSARCINA BARKERI MEDIUM

Overview

External ontology
mediadive.medium:120a (source link unavailable) — METHANOSARCINA BARKERI MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'methanosarcina_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:120', 'original_category': 'archaea'}
  • {'name': 'methanomicrococcus_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:120b', 'original_category': 'archaea'}
  • {'name': 'for_strains_of_methanosarcina_mazei_and_dsm_1538_dsm_11429_dsm_11430_dsm11431_dsm_11432_dsm_11433_and_dsm_11434', 'source': 'KOMODO', 'source_id': 'komodo.medium:120.2', 'original_category': 'archaea'}
  • {'name': 'for_dsm_21571', 'source': 'KOMODO', 'source_id': 'komodo.medium:120.5', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_4556_dsm_10334_and_dsm_13486', 'source': 'KOMODO', 'source_id': 'komodo.medium:120.3', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_9195', 'source': 'KOMODO', 'source_id': 'komodo.medium:120.4', 'original_category': 'bacterial'}
  • {'name': 'g_1_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:120a', 'original_category': 'bacterial'}
  • {'name': 'medium_120_modified_for_dsm_7058', 'source': 'KOMODO', 'source_id': 'komodo.medium:120_7058', 'original_category': 'bacterial'}
  • {'name': 'to_adapt_dsm_804_on_acetate', 'source': 'KOMODO', 'source_id': 'komodo.medium:120.1', 'original_category': 'bacterial'}
pH range
{'min': 6.5, 'max': 6.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120a.pdf

Composition

flowchart LR
    CultureMech_000661["methanosarcina_barkeri_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000661 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000661 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000661 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000661 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000661 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_000661 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_000661 --> ing6_Yeast_extract
    ing7_Casitone["Casitone"]
    CultureMech_000661 --> ing7_Casitone
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000661 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_000661 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Methanol["Methanol"]
    CultureMech_000661 --> ing10_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing10_Methanol -.-> CHEBI_17790
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000661 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000661 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000661 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_H2SO4["H2SO4"]
    CultureMech_000661 --> ing14_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing14_H2SO4 -.-> CHEBI_26836
    ing15_HCl["HCl"]
    CultureMech_000661 --> ing15_HCl
    CHEBI_17883["CHEBI:17883"]
    ing15_HCl -.-> CHEBI_17883
    ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000661 --> ing16_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing17_ZnCl2["ZnCl2"]
    CultureMech_000661 --> ing17_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing17_ZnCl2 -.-> CHEBI_49976
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000661 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_H3BO3["H3BO3"]
    CultureMech_000661 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000661 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000661 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000661 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000661 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_Biotin["Biotin"]
    CultureMech_000661 --> ing24_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_Biotin -.-> CHEBI_15956
    ing25_Folic_acid["Folic acid"]
    CultureMech_000661 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000661 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing27_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000661 --> ing27_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing27_Thiamine_HCl -.-> CHEBI_49105
    ing28_Riboflavin["Riboflavin"]
    CultureMech_000661 --> ing28_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing28_Riboflavin -.-> CHEBI_17015
    ing29_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000661 --> ing29_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing29_Nicotinic_acid -.-> CHEBI_15940
    CultureMech_000661 --> more["...4 more"]

Ingredients (34)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.341797 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.224609 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.488281 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.488281 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.244141 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 NaCl
Cl.Na 58.443 g/mol
2.19727 G_PER_L CHEBI:26710
sodium chloride
7 Yeast extract 1.95312 G_PER_L
8 Casitone 1.95312 G_PER_L
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000488281 G_PER_L CHEBI:8806
Resazurin
10 NaHCO3
CHO3.Na 84.006 g/mol
0.830078 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Methanol
CH4O 32.042 g/mol
15.4688 G_PER_L CHEBI:17790
methanol
12 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.292969 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.292969 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 H2SO4
H2O4S 98.079 g/mol
1000 G_PER_L CHEBI:26836
sulfuric acid
16 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
17 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
18 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
19 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
26 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
27 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
28 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
29 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
30 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
31 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
32 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
33 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
34 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.5 and dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Methanol (50% v/v stock solution) and the reducing agents are each autoclaved separately under 100% N2 gas atmosphere as concentrated solutions in tightly closed tubes. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Appropriate volumes of the solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.5 - 6.8, if necessary.
  2. The ferrous sulfate solution is not stable and should be freshly prepared.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 10 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.227801Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 120a
2026-02-06T05:10:55.027758Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:57.189621Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000661
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.420117+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOSARCINA BARKERI MEDIUM" to "methanosarcina_barkeri_medium" for consistent matching
2026-05-20T06:01:56.275897+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.5-6.8' -> {'min': 6.5, 'max': 6.8}
2026-06-05T07:41:32.377420+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.291445+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:21.987799+00:00 recipe-merger Merged 10 duplicate recipes into canonical record Sources: KOMODO_120_METHANOSARCINA_medium, KOMODO_120b_METHANOMICROCOCCUS_medium, for_strains_of_methanosarcina_mazei_and_dsm_1538_dsm_11429_dsm_11430_dsm11431_dsm_11432_dsm_11433_and_dsm_11434, methanosarcina_barkeri_medium, for_dsm_21571, for_dsm_4556_dsm_10334_and_dsm_13486, for_dsm_9195, g_1_medium, medium_120_modified_for_dsm_7058, to_adapt_dsm_804_on_acetate
2026-08-06T03:04:21.987944+00:00 merge_recipes.py MERGED_RECIPES merged 10 source recipe(s) on fingerprint 328290ecaf2584473fcc69830f5e0c20969a9ff50fa0a70d323faa83649ef16c