marinospirillum_celere

CultureMech:000670 bacterial COMPLEX SOLID_AGAR
Original name: MARINOSPIRILLUM CELERE

Overview

External ontology
mediadive.medium:1212 (source link unavailable) — MARINOSPIRILLUM CELERE
Applications
  • Microbial cultivation
pH
9.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1212.pdf

Composition

flowchart LR
    CultureMech_000670["marinospirillum_celere"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_000670 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000670 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NaCl["NaCl"]
    CultureMech_000670 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Na2SO4["Na2SO4"]
    CultureMech_000670 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000670 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_000670 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_000670 --> ing6_Yeast_extract
    ing7_Peptone["Peptone"]
    CultureMech_000670 --> ing7_Peptone
    ing8_Sodium_DL_lactate["Sodium DL-lactate"]
    CultureMech_000670 --> ing8_Sodium_DL_lactate
    CHEBI_75228["CHEBI:75228"]
    ing8_Sodium_DL_lactate -.-> CHEBI_75228
    ing9_Vitamin_B12["Vitamin B12"]
    CultureMech_000670 --> ing9_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamin_B12 -.-> CHEBI_176843
    ing10_Na2CO3["Na2CO3"]
    CultureMech_000670 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_NaHCO3["NaHCO3"]
    CultureMech_000670 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Agar["Agar"]
    CultureMech_000670 --> ing12_Agar
    CHEBI_2509["CHEBI:2509"]
    ing12_Agar -.-> CHEBI_2509
    ing13_HCl["HCl"]
    CultureMech_000670 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000670 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_ZnCl2["ZnCl2"]
    CultureMech_000670 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000670 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_000670 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000670 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000670 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000670 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000670 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
4 Na2SO4
2Na.O4S 142.043 g/mol
0.5 G_PER_L CHEBI:32149
sodium sulfate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
7 Yeast extract 1 G_PER_L
8 Peptone 1 G_PER_L
9 Sodium DL-lactate
C3H5O3.Na 112.06 g/mol
1 G_PER_L CHEBI:75228
sodium lactate
10 Vitamin B12 1.5e-05 G_PER_L CHEBI:176843
vitamin B12
11 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
12 NaHCO3
CHO3.Na 84.006 g/mol
5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
13 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
14 HCl
HCl 36.461 g/mol
1.925 G_PER_L CHEBI:17883
hydrogen chloride
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.4881 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.0694444 G_PER_L CHEBI:49976
zinc dichloride
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0992064 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.297619 G_PER_L CHEBI:33118
boric acid
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.188492 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.00198413 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0238095 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0357143 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Autoclave the Na2CO3, NaHCO3 and the lactate separately from the medium and combine after cooling. Vitamin B12 is added from a sterile stock solution. The final pH of the medium should be 9.5. Add 15.0 g/l agar for solid media.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:13.729367Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1212
2026-03-13T06:18:57.324399Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000670
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.580222+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MARINOSPIRILLUM CELERE" to "marinospirillum_celere" for consistent matching
2026-06-05T07:41:13.188208+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.982942+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:42.180630+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2ed9350ce23aecd24d5b7105a0e154b72cbbb4a450b43addcd96fefb703edcd6