nsc3_medium_with_acetate

CultureMech:000705 bacterial DEFINED SOLID_AGAR
Original name: NSC3-MEDIUM WITH ACETATE

Overview

External ontology
mediadive.medium:1246 (source link unavailable) — NSC3-MEDIUM WITH ACETATE
Applications
  • Microbial cultivation
pH range
{'min': 8.5, 'max': 9.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1246.pdf

Composition

flowchart LR
    CultureMech_000705["nsc3_medium_with_acetate"]
    ing0_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000705 --> ing0_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing0_Na2S_x_9_H2O -.-> CHEBI_76209
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000705 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000705 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NaHCO3["NaHCO3"]
    CultureMech_000705 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_NaCl["NaCl"]
    CultureMech_000705 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na_acetate["Na-acetate"]
    CultureMech_000705 --> ing5_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Na_acetate -.-> CHEBI_32954
    ing6_Agar["Agar"]
    CultureMech_000705 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_EDTA["EDTA"]
    CultureMech_000705 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000705 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000705 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000705 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_000705 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000705 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000705 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000705 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000705 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
1 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
3 K2HPO4
HO4P.2K 174.174 g/mol
0.25 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 NaHCO3
CHO3.Na 84.006 g/mol
1.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
6 Na-acetate
C2H3O2.Na 82.034 g/mol
0.44 G_PER_L CHEBI:32954
sodium acetate
7 Agar 15 G_PER_L CHEBI:2509
agar
8 EDTA
C10H16N2O8 292.244 g/mol
0.5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 8.5-9.0 before autoclaving.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:13.844681Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1246
2026-03-13T06:18:57.630374Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000705
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:08.940026+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NSC3-MEDIUM WITH ACETATE" to "nsc3_medium_with_acetate" for consistent matching
2026-05-20T06:02:01.271635+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '8.5-9' -> {'min': 8.5, 'max': 9.0}
2026-06-05T07:41:40.085721+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.689799+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:51.635397+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0d6400cd5f9564b2ac829e124498b95bf0cd823688dec17fd729a1455d144016