Overview
- External ontology
- mediadive.medium:1248 (source link unavailable) — KOSMOTOGA SHENGLIENSIS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'thermococcoides_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1248', 'original_category': 'bacterial'}
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1248.pdf
Composition
flowchart LR
CultureMech_000707["kosmotoga_shengliensis_medium"]
ing0_NaCl["NaCl"]
CultureMech_000707 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_000707 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000707 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_K2HPO4["K2HPO4"]
CultureMech_000707 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_NH4Cl["NH4Cl"]
CultureMech_000707 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Na_acetate["Na-acetate"]
CultureMech_000707 --> ing5_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Na_acetate -.-> CHEBI_32954
ing6_Yeast_extract["Yeast extract"]
CultureMech_000707 --> ing6_Yeast_extract
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_000707 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000707 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_000707 --> ing9_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing9_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing10_D_Glucose["D-Glucose"]
CultureMech_000707 --> ing10_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing10_D_Glucose -.-> CHEBI_17634
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
18 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.34 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.45 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 | Yeast extract | 2 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 11 |
D-Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17634
D-glucose
|
Preparation
- Dissolve ingredients except cysteine, thiosulfate and glucose. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and adjust pH to 6.8 - 7.2. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate and glucose from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- kosmotoga_shengliensis_medium (CultureMech:000707)
- thermococcoides_medium (CultureMech:004005)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.849267Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1248 |
| 2026-03-13T06:18:57.640267Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000707 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:08.951699+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KOSMOTOGA SHENGLIENSIS MEDIUM" to "kosmotoga_shengliensis_medium" for consistent matching |
| 2026-06-05T07:41:10.554204+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.427847+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.784404+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: kosmotoga_shengliensis_medium, thermococcoides_medium |
| 2026-08-06T03:05:39.784454+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint c13121b40d05895c32a5c978d35be00594ad1119126cc37cfa5182e4f9472871 |