medium_vl55

CultureMech:000725 bacterial DEFINED SOLID_AGAR
Original name: MEDIUM VL55

Overview

External ontology
mediadive.medium:1266 (source link unavailable) — MEDIUM VL55
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1266.pdf

Composition

flowchart LR
    CultureMech_000725["medium_vl55"]
    ing0_MES["MES"]
    CultureMech_000725 --> ing0_MES
    CHEBI_39010["CHEBI:39010"]
    ing0_MES -.-> CHEBI_39010
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000725 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000725 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3__NH4_2HPO4["(NH4)2HPO4"]
    CultureMech_000725 --> ing3__NH4_2HPO4
    CHEBI_63051["CHEBI:63051"]
    ing3__NH4_2HPO4 -.-> CHEBI_63051
    ing4_Glucose["Glucose"]
    CultureMech_000725 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Agar["Agar"]
    CultureMech_000725 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_NaOH["NaOH"]
    CultureMech_000725 --> ing6_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing6_NaOH -.-> CHEBI_32145
    ing7_KOH["KOH"]
    CultureMech_000725 --> ing7_KOH
    CHEBI_32035["CHEBI:32035"]
    ing7_KOH -.-> CHEBI_32035
    ing8_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000725 --> ing8_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing8_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing9_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000725 --> ing9_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing9_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing10_HCl["HCl"]
    CultureMech_000725 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000725 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_000725 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000725 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_000725 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000725 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000725 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000725 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000725 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Vitamin_B12["Vitamin B12"]
    CultureMech_000725 --> ing19_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing19_Vitamin_B12 -.-> CHEBI_176843
    ing20_4_aminobenzoate["4-aminobenzoate"]
    CultureMech_000725 --> ing20_4_aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing20_4_aminobenzoate -.-> CHEBI_17836
    ing21_Biotin["Biotin"]
    CultureMech_000725 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000725 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Hemicalcium_D_____pantothenate["Hemicalcium D-(+)-pantothenate"]
    CultureMech_000725 --> ing23_Hemicalcium_D_____pantothenate
    ing24_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_000725 --> ing24_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing24_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing25_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000725 --> ing25_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing25_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing26_DL_6_8_thioctic_acid["DL-6,8-thioctic acid"]
    CultureMech_000725 --> ing26_DL_6_8_thioctic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26_DL_6_8_thioctic_acid -.-> CHEBI_16494
    ing27_Riboflavin["Riboflavin"]
    CultureMech_000725 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Folic_acid["Folic acid"]
    CultureMech_000725 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 MES 5.85 G_PER_L CHEBI:39010
MES
 [Merged 2 duplicates: 1.95, 3.9]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
5.047885600000001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 3 duplicates: 0.0492952, 0.0985904, 4.9]
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
4.532313500000001 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 3 duplicates: 0.0441045, 0.088209, 4.4]
4 (NH4)2HPO4
2H4N.HO4P 132.056 g/mol
2.679236 G_PER_L CHEBI:63051
diammonium hydrogen phosphate
 [Merged 3 duplicates: 0.026412, 0.052824, 2.6]
5 Glucose
C6H12O6 180.156 g/mol
0.72064 G_PER_L CHEBI:17234
glucose
 [Merged 2 duplicates: 0.36032, 0.36032]
6 Agar 7.5 G_PER_L CHEBI:2509
agar
7 NaOH
HO.Na 39.997 g/mol
8.5 G_PER_L CHEBI:32145
sodium hydroxide
 [Merged 2 duplicates: 8.0, 0.5]
8 KOH
HO.K 56.105 g/mol
5.6 G_PER_L CHEBI:32035
potassium hydroxide
9 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
10 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
11 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
12 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Vitamin B12 0.017 G_PER_L CHEBI:176843
vitamin B12
21 4-aminobenzoate
C7H6NO2 136.13 g/mol
0.013 G_PER_L CHEBI:17836
4-aminobenzoate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.003 G_PER_L CHEBI:15956
biotin
23 Nicotinic acid
C6H5NO2 123.111 g/mol
0.033 G_PER_L CHEBI:15940
nicotinic acid
24 Hemicalcium D-(+)-pantothenate 0.017 G_PER_L
25 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.05 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
26 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.033 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
27 DL-6,8-thioctic acid
C8H14O2S2 206.332 g/mol
0.01 G_PER_L CHEBI:16494
lipoic acid
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid

Preparation

  1. Adjust to pH 5.5 with NaOH/KOH solution (see below).
  2. After autoclaving add:
  3. Adjust to pH 5.5 with NaOH/KOH solution (see below).
  4. After autoclaving, keep at about 60°C, then add to each 250 ml lot:
  5. Autoclave in 250ml lots, with a magnetic stirring bar in each bottle.
  6. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  7. Filter sterilize the solution.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:13.898044Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1266
2026-03-13T03:08:39.834308Z data-quality-cleanup-v1.0 Data quality fixes Merged 9 duplicate ingredient(s)
2026-03-13T06:18:57.800301Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000725
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.123899+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM VL55" to "medium_vl55" for consistent matching
2026-06-05T07:41:30.263369+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.443379+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:06.501346+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.307200+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 22c17dbce91946e7824fb5e97013b8cbc5799fbb433f51201e13edae320904ef