Overview
- External ontology
- mediadive.medium:1276 (source link unavailable) — MEDIUM FOR CHELATOCOCCUS BUHARII
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1276.pdf
Composition
flowchart LR
CultureMech_000737["medium_for_chelatococcus_buharii"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000737 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_000737 --> ing1_Tryptone
ing2_Nitrilotriacetate["Nitrilotriacetate"]
CultureMech_000737 --> ing2_Nitrilotriacetate
CHEBI_25548["CHEBI:25548"]
ing2_Nitrilotriacetate -.-> CHEBI_25548
ing3_K2HPO4["K2HPO4"]
CultureMech_000737 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
CultureMech_000737 --> ing4_NaH2PO4_x_2_H2O
CHEBI_37585["CHEBI:37585"]
ing4_NaH2PO4_x_2_H2O -.-> CHEBI_37585
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000737 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_000737 --> ing6_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing6_CaSO4_x_2_H2O -.-> CHEBI_32583
ing7_Fe_III__citrate["Fe(III) citrate"]
CultureMech_000737 --> ing7_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing7_Fe_III__citrate -.-> CHEBI_144421
ing8_Agar["Agar"]
CultureMech_000737 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000737 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000737 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_000737 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000737 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000737 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000737 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000737 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2.5 G_PER_L | ||
| 2 | Tryptone | 2.5 G_PER_L | ||
| 3 |
Nitrilotriacetate
C6H6NO6 188.115 g/mol
|
1 G_PER_L |
CHEBI:25548
nitrilotriacetate(3-)
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.27 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
|
0.54 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.4 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 8 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.5 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 9 | Agar | 18 G_PER_L |
CHEBI:2509
agar
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Before adding agar, heat to boiling. After cooling adjust pH to pH 7.5. (pH drops strongly during heating).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_for_chelatococcus_buharii (CultureMech:000737)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.957514Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1276 |
| 2026-03-13T06:18:57.936994Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000737 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.275210+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM FOR CHELATOCOCCUS BUHARII" to "medium_for_chelatococcus_buharii" for consistent matching |
| 2026-06-05T07:41:28.928009+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.005719+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.063194+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 394c595e8c858648b05078130c2818b3cee73afa8c24eecb2f8353c359c077ca |