Overview
- External ontology
- mediadive.medium:1279 (source link unavailable) — HELICOBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1279.pdf
Composition
flowchart LR
CultureMech_000742["helicobacter_medium"]
ing0_Brucella_Broth["Brucella Broth"]
CultureMech_000742 --> ing0_Brucella_Broth
ing1_Demineralized_water["Demineralized water"]
CultureMech_000742 --> ing1_Demineralized_water
CHEBI_15377["CHEBI:15377"]
ing1_Demineralized_water -.-> CHEBI_15377
ing2_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_000742 --> ing2_Fetal_bovine_serum
ing3_Vitox["Vitox"]
CultureMech_000742 --> ing3_Vitox
ing4_Skirrow_supplement["Skirrow supplement"]
CultureMech_000742 --> ing4_Skirrow_supplement
ing5_Amphotericin["Amphotericin"]
CultureMech_000742 --> ing5_Amphotericin
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Brucella Broth | 35 G_PER_L | ||
| 2 |
Demineralized water
H2O 18.015 g/mol
|
80 G_PER_L |
CHEBI:15377
water
|
|
| 3 | Fetal bovine serum | 2 G_PER_L | ||
| 4 | Vitox | 0.06 G_PER_L | ||
| 5 | Skirrow supplement | 0.02 G_PER_L | ||
| 6 | Amphotericin | 0.02 G_PER_L |
Preparation
- Adjust with HCl to pH 5.0, make anoxic under a stream of N2/CO2, distribute in Hungate tubes to 8 ml and autoclave.
- Add aseptically to each tube:
- Add 1 ml of sterile air to each tube.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- helicobacter_medium (CultureMech:000742)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.978909Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1279 |
| 2026-03-13T06:18:57.999704Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000742 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.344535+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HELICOBACTER MEDIUM" to "helicobacter_medium" for consistent matching |
| 2026-06-05T07:41:08.345658+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:37.446574+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7e6ae7340f276d403b937303afeddda23a500d07bf01338bb1056ea95119692d |