Overview
- External ontology
- mediadive.medium:1281 (source link unavailable) — ANAEROCELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1281.pdf
Composition
flowchart LR
CultureMech_000745["anaerocella_medium"]
ing0_Trypticase["Trypticase"]
CultureMech_000745 --> ing0_Trypticase
ing1_Yeast_extract["Yeast extract"]
CultureMech_000745 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_000745 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Cellobiose["Cellobiose"]
CultureMech_000745 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Maltose["Maltose"]
CultureMech_000745 --> ing4_Maltose
CHEBI_17306["CHEBI:17306"]
ing4_Maltose -.-> CHEBI_17306
ing5_Starch["Starch"]
CultureMech_000745 --> ing5_Starch
CHEBI_28017["CHEBI:28017"]
ing5_Starch -.-> CHEBI_28017
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000745 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Resazurin["Resazurin"]
CultureMech_000745 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Na2CO3["Na2CO3"]
CultureMech_000745 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Agar["Agar"]
CultureMech_000745 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_K2HPO4["K2HPO4"]
CultureMech_000745 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_KH2PO4["KH2PO4"]
CultureMech_000745 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12__NH4_2SO4["(NH4)2SO4"]
CultureMech_000745 --> ing12__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing12__NH4_2SO4 -.-> CHEBI_62946
ing13_NaCl["NaCl"]
CultureMech_000745 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000745 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000745 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000745 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_D_____biotin["D-(+)-biotin"]
CultureMech_000745 --> ing17_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing17_D_____biotin -.-> CHEBI_15956
ing18_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000745 --> ing18_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase | 10 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 5 |
Maltose
C12H22O11 342.297 g/mol
|
0.25 G_PER_L |
CHEBI:17306
maltose
|
|
| 6 | Starch | 0.25 G_PER_L |
CHEBI:28017
starch
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.2 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
12 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Preparation
- Boil 10 minutes under N2 stream, cool to room temperature. Adjust pH to 7.3 and autoclave for 20 minutes at 121°C. After autoclaving add sterile, anoxic solutions marked with #. For preparation of solid medium add 15.0 g agar per liter.
Solutions
Vitamin solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 0.1 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | |
| Pyridoxine hydrochloride | 0.3 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- anaerocella_medium (CultureMech:000745)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.986141Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1281 |
| 2026-01-28T19:48:36.307116Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.027024Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000745 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.375314+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROCELLA MEDIUM" to "anaerocella_medium" for consistent matching |
| 2026-06-05T07:40:49.024137+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.255150+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T07:05:21.610722+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-09-22T00:09:29.404042+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 96763287bf195de1fd8e51e6f8e260279e96a23507292ed759b3088b8ac03617 |