butyrivibrio_m2_medium

Overview

External ontology

mediadive.medium:1310 (source link unavailable) — BUTYRIVIBRIO M2 MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'm2_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:1310', 'original_category': 'bacterial'}

pH range

{'min': 6.5, 'max': 6.8}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1310.pdf

Composition

flowchart LR
    CultureMech_000772["butyrivibrio_m2_medium"]
    ing0_Casitone["Casitone"]
    CultureMech_000772 --> ing0_Casitone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000772 --> ing1_Yeast_extract
    ing2_D_Glucose["D-Glucose"]
    CultureMech_000772 --> ing2_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D_Glucose -.-> CHEBI_17634
    ing3_Cellobiose["Cellobiose"]
    CultureMech_000772 --> ing3_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing3_Cellobiose -.-> CHEBI_17057
    ing4_Maltose["Maltose"]
    CultureMech_000772 --> ing4_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing4_Maltose -.-> CHEBI_17306
    ing5_Na_DL_lactate["Na-DL-lactate"]
    CultureMech_000772 --> ing5_Na_DL_lactate
    CHEBI_75228["CHEBI:75228"]
    ing5_Na_DL_lactate -.-> CHEBI_75228
    ing6_K2HPO4["K2HPO4"]
    CultureMech_000772 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_KH2PO4["KH2PO4"]
    CultureMech_000772 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000772 --> ing8__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing8__NH4_2SO4 -.-> CHEBI_62946
    ing9_NaCl["NaCl"]
    CultureMech_000772 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000772 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000772 --> ing11_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing12_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000772 --> ing12_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Sodium_resazurin -.-> CHEBI_8806
    ing13_Na2CO3["Na2CO3"]
    CultureMech_000772 --> ing13_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing13_Na2CO3 -.-> CHEBI_29377
    ing14_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000772 --> ing14_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing14_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000772 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (16)

Preparation

1. Dissolve ingredients (except carbonate, cysteine and sulfide), boil medium for 1 min, then cool to room temperature under 100% CO2 gas atmosphere. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Add cysteine and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of the complete medium to 6.5 - 6.8, if necessary.
2. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• KOMODO_1310_M2_medium (CultureMech:004067)
• butyrivibrio_m2_medium (CultureMech:000772)