methyloferula_stellata_medium

CultureMech:000779 bacterial DEFINED LIQUID
Original name: METHYLOFERULA STELLATA MEDIUM

Overview

External ontology
mediadive.medium:1322 (source link unavailable) — METHYLOFERULA STELLATA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 5.0, 'max': 5.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1322.pdf

Composition

flowchart LR
    CultureMech_000779["methyloferula_stellata_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000779 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000779 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000779 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000779 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaCl["NaCl"]
    CultureMech_000779 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Methanol["Methanol"]
    CultureMech_000779 --> ing5_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing5_Methanol -.-> CHEBI_17790
    ing6_EDTA["EDTA"]
    CultureMech_000779 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_000779 --> ing7_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing7_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000779 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000779 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000779 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000779 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_Na2MoO4["Na2MoO4"]
    CultureMech_000779 --> ing12_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing12_Na2MoO4 -.-> CHEBI_75215
    ing13_Na2_EDTA["Na2-EDTA"]
    CultureMech_000779 --> ing13_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing13_Na2_EDTA -.-> CHEBI_64734

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.1 G_PER_L CHEBI:62946
ammonium sulfate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NaCl
Cl.Na 58.443 g/mol
0.02 G_PER_L CHEBI:26710
sodium chloride
6 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
7 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.1 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
3.54 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 2.0, 1.54]
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 Na2MoO4
MoO4.2Na 205.916 g/mol
0.03 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
14 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
2.06 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)

Preparation

  1. Final pH 5.0-5.8.
  2. Prepare the medium without methanol in closed vessels. Adjust pH to 5.0-5.8 and sterilize. Allow to cool to about 30°C before adding 10 mL filter-sterilized methanol per litre medium.
  3. The medium is fairly weakly buffered so the pH should be checked before and after autoclaving. The pH should be adjusted with H3PO4 (sterilised if added to sterile medium). The strain should be grown with shaking.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.094197Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1322
2026-03-13T03:08:48.099777Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:58.299716Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000779
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.691799+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOFERULA STELLATA MEDIUM" to "methyloferula_stellata_medium" for consistent matching
2026-05-20T06:01:56.803793+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5-5.8' -> {'min': 5.0, 'max': 5.8}
2026-06-05T07:41:33.159612+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.570648+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.885366+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4fc16e2ea61816c1e02397a91f5820f69a257bc4731b7aa238d941ba38f329d8